Human serum catalase decreases endothelial cell injury from hydrogen peroxide

J. A. Leff, M. A. Oppegard, L. S. Terada, E. C. McCarty, J. E. Repine

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Serum from normal human subjects contained variable amounts of catalase activity, which was inhibitable by heat, azide, trichloroacetic acid (TCA), or aminotriazole treatment. Serum also decreased hydrogen peroxide (H2O2) concentrations in vitro and H2O2-mediated injury to cultured endothelial cells. By comparison, heat-, azide-, TCA-, or aminotriazole-treated serum neither decreased H2O2 concentrations in vitro nor reduced H2O2-mediated damage to endothelial cells. We conclude that serum catalase activity can alter H2O2-dependent reactions. We speculate that variations in serum catalase activity may alter individual susceptibility to oxidant-mediated vascular disease or be a factor when added to test systems in vitro.

Original languageEnglish (US)
Pages (from-to)1903-1906
Number of pages4
JournalJournal of Applied Physiology
Volume71
Issue number5
StatePublished - 1991

Fingerprint

Catalase
Hydrogen Peroxide
Endothelial Cells
Amitrole
Wounds and Injuries
Serum
Trichloroacetic Acid
Azides
Hot Temperature
Vascular Diseases
Oxidants
Cultured Cells
In Vitro Techniques

Keywords

  • antioxidants
  • oxygen radicals
  • peroxide
  • vascular injury

ASJC Scopus subject areas

  • Endocrinology
  • Physiology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Leff, J. A., Oppegard, M. A., Terada, L. S., McCarty, E. C., & Repine, J. E. (1991). Human serum catalase decreases endothelial cell injury from hydrogen peroxide. Journal of Applied Physiology, 71(5), 1903-1906.

Human serum catalase decreases endothelial cell injury from hydrogen peroxide. / Leff, J. A.; Oppegard, M. A.; Terada, L. S.; McCarty, E. C.; Repine, J. E.

In: Journal of Applied Physiology, Vol. 71, No. 5, 1991, p. 1903-1906.

Research output: Contribution to journalArticle

Leff, JA, Oppegard, MA, Terada, LS, McCarty, EC & Repine, JE 1991, 'Human serum catalase decreases endothelial cell injury from hydrogen peroxide', Journal of Applied Physiology, vol. 71, no. 5, pp. 1903-1906.
Leff, J. A. ; Oppegard, M. A. ; Terada, L. S. ; McCarty, E. C. ; Repine, J. E. / Human serum catalase decreases endothelial cell injury from hydrogen peroxide. In: Journal of Applied Physiology. 1991 ; Vol. 71, No. 5. pp. 1903-1906.
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