Human spasmolytic polypeptide decreases proton permeation through gastric mucus in vivo and in vitro

Shin Tanaka, Daniel K. Podolsky, Eli Engel, Paul H. Guth, Jonathan D. Kaunitz

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Exogenously administered trefoil peptides are gastroprotective in rat injury models. We hypothesized that trefoil-associated gastropretection occurred by decreasing the rate of proton permeation through mucus. Gastric surface cell intracellular pH and mucus gel thickness were measured by in vive microscopy. Gastric mucosal blood flow was measured by laser-Doppler flowmetry. The effect of human spasmolytic peptide (hSP) on H+ diffusion through 5% purified porcine mucin was measured using an Ussing chamber. Buffering action of mucin was measured by titration. In vitro, gastric mucosal blood flow and mucus gel thickness were not affected by any of the treatments. Topical hSP, but not intravenous hSP, decreased initial acidification rate and elevated the intracellular pH of gastric surface cells during luminal acid challenge. In in vitro studies, hSP dose dependently decreased the diffusion coefficient of H+ through 5% porcine mucin solution. hSP had no significant effect on the buffering action of mucin solutions. These data support our hypothesis that hSP interacts with gastric mucin in a manner that inhibits proton permeation through the mucus gel layer.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume272
Issue number6 35-6
StatePublished - Jun 1997

Fingerprint

Parasympatholytics
Mucus
Protons
Stomach
Mucins
Peptides
Gels
Swine
Gastric Mucins
Laser-Doppler Flowmetry
In Vitro Techniques
spasmolytic polypeptide
Microscopy
Acids
Wounds and Injuries

Keywords

  • Fluorescein dyes
  • In vivo microscopy
  • Intracellular pH
  • Stomach
  • Trefoil peptides

ASJC Scopus subject areas

  • Gastroenterology
  • Physiology
  • Physiology (medical)

Cite this

Human spasmolytic polypeptide decreases proton permeation through gastric mucus in vivo and in vitro. / Tanaka, Shin; Podolsky, Daniel K.; Engel, Eli; Guth, Paul H.; Kaunitz, Jonathan D.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 272, No. 6 35-6, 06.1997.

Research output: Contribution to journalArticle

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