Human surfactant protein B promoter in transgenic mice: Temporal, spatial, and stimulus-responsive regulation

Marlene Strayer, Rashmin C. Savani, Linda W. Gonzales, Aisha Zaman, Zheng Cui, Edina Veszelovszky, Emily Wood, Ye Shih Ho, Philip L. Ballard

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Surfactant protein B (SP-B) is a developmentally and hormonally regulated lung protein that is required for normal surfactant function. We generated transgenic mice carrying the human SP-B promoter (-1,039/+431 bp) linked to chloramphenicol acetyltransferase (CAT). CAT activity was high in lung and immunoreactive protein localized to alveolar type II and bronchiolar epithehal cells. In addition, thyroid, trachea, and intestine demonstrated CAT activity, and each of these tissues also expressed low levels of SP-B mRNA. Developmental expression of CAT activity and SP-B mRNA in fetal lung were similar and both increased during explant culture. SP-B mRNA but not CAT activity decreased during culture of adult lung, and both were reduced by transforming growth factor (TGF)-β1. Treatment of adult mice with intratracheal bleomycin caused similar time-dependent decreases in lung SP-B mRNA and CAT activity. These findings indicate that the human SP-B promoter fragment directs tissue- and lung cell-specific transgene expression and contains cis-acting elements involved in regulated expression during development, fetal lung explant culture, and responsiveness to TGF-β and bleomycin-induced lung injury.

Original languageEnglish (US)
Pages (from-to)L394-L404
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume282
Issue number3 26-3
DOIs
StatePublished - 2002

Keywords

  • Bleomycin lung injury
  • Dexamethasone
  • Lung development
  • Lung explant culture
  • Transforming growth factor-β

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

Fingerprint Dive into the research topics of 'Human surfactant protein B promoter in transgenic mice: Temporal, spatial, and stimulus-responsive regulation'. Together they form a unique fingerprint.

  • Cite this