Human T-cell leukemia virus types I and II exhibit different DNase I protection patterns

R. Altman, D. Harrich, J. A. Garcia, R. B. Gaynor

Research output: Contribution to journalArticle

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Abstract

Human T-cell leukemia virus types I (HTLV-I) and II (HTLV-II) are human retroviruses which normally infect T-lymphoid cells. HTLV-I infection is associated with adult T-cell leukemia-lymphoma, and HTLV-II is associated with an indolent form of hairy-cell leukemia. To identify potential transcriptional regulatory elements of these two related human retroviruses, we performed DNase I footprinting of both the HTLV-I and HTLV-II long terminal repeats (LTRs) by using extracts prepared from uninfected T cells, HTLV-I and HTLV-II transformed T cells, and HeLa cells. Five regions of the HTLV-I LTR and three regions of the HTLV-II LTR showed protection by DNase I footprinting. All three of the 21-base-pair repeats previously shown to be important in HTLV transcriptional regulation were protected in the HTLV-I LTR, whereas only one of these repeats was protected in the HTLV-II LTR. Several regions exhibited altered protection in extracts prepared from lymphoid cells as compared with HeLa cells, but there were minimal differences in the protection patterns between HTLV-infected and uninfected lymphoid extracts. A number of HTLV-I and HTLV-II LTR fragments which contained regions showing protection in DNase I footprinting were able to function as inducible enhancer elements in transient CAT gene expression assays in the presence of the HTLV-II tat protein. The alterations in the pattern of the cellular proteins which bind to thew HTLV-I and HTLV-II LTRs may in part be responsible for differences in the transcriptional regulation of these two related viruses.

Original languageEnglish (US)
Pages (from-to)1339-1346
Number of pages8
JournalJournal of Virology
Volume62
Issue number4
StatePublished - 1988

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Primate T-lymphotropic virus 1
Human T-lymphotropic virus 2
deoxyribonuclease I
Human T-lymphotropic virus 1
Deoxyribonuclease I
terminal repeat sequences
Terminal Repeat Sequences
Retroviridae
T-lymphocytes
leukemia
cells
extracts
enhancer elements
lymphoma
HeLa Cells
proteins
Transcriptional Regulatory Elements
Lymphocytes
tat Gene Products
T-Lymphocytes

ASJC Scopus subject areas

  • Immunology

Cite this

Human T-cell leukemia virus types I and II exhibit different DNase I protection patterns. / Altman, R.; Harrich, D.; Garcia, J. A.; Gaynor, R. B.

In: Journal of Virology, Vol. 62, No. 4, 1988, p. 1339-1346.

Research output: Contribution to journalArticle

Altman, R. ; Harrich, D. ; Garcia, J. A. ; Gaynor, R. B. / Human T-cell leukemia virus types I and II exhibit different DNase I protection patterns. In: Journal of Virology. 1988 ; Vol. 62, No. 4. pp. 1339-1346.
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