Human TOB, an antiproliferative transcription factor, is a poly(A)-binding protein-dependent positive regulator of cytoplasmic mRNA deadenylation

Nader Ezzeddine, Tsung Cheng Chang, Wenmiao Zhu, Akio Yamashita, Chyi Ying A Chen, Zhenping Zhong, Yukiko Yamashita, Dinghai Zheng, Ann Bin Shyu

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106 Citations (Scopus)

Abstract

In mammalian cells, mRNA decay begins with deadenylation, which involves two consecutive phases mediated by the PAN2-PAN3 and the CCR4-CAF1 complexes, respectively. The regulation of the critical deadenylation step and its relationship with RNA-processing bodies (P-bodies), which are thought to be a site where poly(A)-shortened mRNAs get degraded, are poorly understood. Using the Tet-Off transcriptional pulsing approach to investigate mRNA decay in mouse NIH 3T3 fibroblasts, we found that TOB, an antiproliferative transcription factor, enhances mRNA deadenylation in vivo. Results from glutathione S-transferase pull-down and coimmunoprecipitation experiments indicate that TOB can simultaneously interact with the poly(A) nuclease complex CCR4-CAF1 and the cytoplasmic poly(A)-binding protein, PABPC1. Combining these findings with those from mutagenesis studies, we further identified the protein motifs on TOB and PABPC1 that are necessary for their interaction and found that interaction with PABPC1 is necessary for TOB's deadenylation-enhancing effect. Moreover, our immunofluorescence microscopy results revealed that TOB colocalizes with P-bodies, suggesting a role of TOB in linking deadenylation to the P-bodies. Our findings reveal a new mechanism by which the fate of mammalian mRNA is modulated at the deadenylation step by a protein that recruits poly(A) nuclease(s) to the 3′ poly(A) tail-PABP complex.

Original languageEnglish (US)
Pages (from-to)7791-7801
Number of pages11
JournalMolecular and Cellular Biology
Volume27
Issue number22
DOIs
StatePublished - Nov 2007

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Poly(A)-Binding Proteins
Transcription Factors
Messenger RNA
RNA Stability
Amino Acid Motifs
Glutathione Transferase
Fluorescence Microscopy
Mutagenesis
Fibroblasts
RNA
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Human TOB, an antiproliferative transcription factor, is a poly(A)-binding protein-dependent positive regulator of cytoplasmic mRNA deadenylation. / Ezzeddine, Nader; Chang, Tsung Cheng; Zhu, Wenmiao; Yamashita, Akio; Chen, Chyi Ying A; Zhong, Zhenping; Yamashita, Yukiko; Zheng, Dinghai; Shyu, Ann Bin.

In: Molecular and Cellular Biology, Vol. 27, No. 22, 11.2007, p. 7791-7801.

Research output: Contribution to journalArticle

Ezzeddine, Nader ; Chang, Tsung Cheng ; Zhu, Wenmiao ; Yamashita, Akio ; Chen, Chyi Ying A ; Zhong, Zhenping ; Yamashita, Yukiko ; Zheng, Dinghai ; Shyu, Ann Bin. / Human TOB, an antiproliferative transcription factor, is a poly(A)-binding protein-dependent positive regulator of cytoplasmic mRNA deadenylation. In: Molecular and Cellular Biology. 2007 ; Vol. 27, No. 22. pp. 7791-7801.
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abstract = "In mammalian cells, mRNA decay begins with deadenylation, which involves two consecutive phases mediated by the PAN2-PAN3 and the CCR4-CAF1 complexes, respectively. The regulation of the critical deadenylation step and its relationship with RNA-processing bodies (P-bodies), which are thought to be a site where poly(A)-shortened mRNAs get degraded, are poorly understood. Using the Tet-Off transcriptional pulsing approach to investigate mRNA decay in mouse NIH 3T3 fibroblasts, we found that TOB, an antiproliferative transcription factor, enhances mRNA deadenylation in vivo. Results from glutathione S-transferase pull-down and coimmunoprecipitation experiments indicate that TOB can simultaneously interact with the poly(A) nuclease complex CCR4-CAF1 and the cytoplasmic poly(A)-binding protein, PABPC1. Combining these findings with those from mutagenesis studies, we further identified the protein motifs on TOB and PABPC1 that are necessary for their interaction and found that interaction with PABPC1 is necessary for TOB's deadenylation-enhancing effect. Moreover, our immunofluorescence microscopy results revealed that TOB colocalizes with P-bodies, suggesting a role of TOB in linking deadenylation to the P-bodies. Our findings reveal a new mechanism by which the fate of mammalian mRNA is modulated at the deadenylation step by a protein that recruits poly(A) nuclease(s) to the 3′ poly(A) tail-PABP complex.",
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AU - Chang, Tsung Cheng

AU - Zhu, Wenmiao

AU - Yamashita, Akio

AU - Chen, Chyi Ying A

AU - Zhong, Zhenping

AU - Yamashita, Yukiko

AU - Zheng, Dinghai

AU - Shyu, Ann Bin

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