Human x mouse hybrid cells segregating mouse chromosomes and isozymes

John D. Minna, Hayden G. Coon

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

THE usual pattern of chromosome segregation in interspecific human×mouse somatic cell hybrids is retention of mouse and loss of human chromosomes1. In attempts to generate hybrid strains expressing differentiated functions, we have fused an actively dividing human cell line with freshly dissociated, highly differentiated cells from the mouse embryonic nervous system. The majority of the resulting hybrid strains retain human while losing mouse chromosomes and isozymes and thus provide a new class of hybrid cell for analysis of gene linkage and regulation.

Original languageEnglish (US)
Pages (from-to)401-404
Number of pages4
JournalNature
Volume252
Issue number5482
DOIs
StatePublished - 1974

Fingerprint

Hybrid Cells
Isoenzymes
Chromosomes
Chromosome Segregation
Nervous System
Cell Line
Genes

ASJC Scopus subject areas

  • General

Cite this

Human x mouse hybrid cells segregating mouse chromosomes and isozymes. / Minna, John D.; Coon, Hayden G.

In: Nature, Vol. 252, No. 5482, 1974, p. 401-404.

Research output: Contribution to journalArticle

Minna, John D. ; Coon, Hayden G. / Human x mouse hybrid cells segregating mouse chromosomes and isozymes. In: Nature. 1974 ; Vol. 252, No. 5482. pp. 401-404.
@article{a45648ba4eac4e4f894545486b20a299,
title = "Human x mouse hybrid cells segregating mouse chromosomes and isozymes",
abstract = "THE usual pattern of chromosome segregation in interspecific human×mouse somatic cell hybrids is retention of mouse and loss of human chromosomes1. In attempts to generate hybrid strains expressing differentiated functions, we have fused an actively dividing human cell line with freshly dissociated, highly differentiated cells from the mouse embryonic nervous system. The majority of the resulting hybrid strains retain human while losing mouse chromosomes and isozymes and thus provide a new class of hybrid cell for analysis of gene linkage and regulation.",
author = "Minna, {John D.} and Coon, {Hayden G.}",
year = "1974",
doi = "10.1038/252401a0",
language = "English (US)",
volume = "252",
pages = "401--404",
journal = "Nature",
issn = "0028-0836",
publisher = "Nature Publishing Group",
number = "5482",

}

TY - JOUR

T1 - Human x mouse hybrid cells segregating mouse chromosomes and isozymes

AU - Minna, John D.

AU - Coon, Hayden G.

PY - 1974

Y1 - 1974

N2 - THE usual pattern of chromosome segregation in interspecific human×mouse somatic cell hybrids is retention of mouse and loss of human chromosomes1. In attempts to generate hybrid strains expressing differentiated functions, we have fused an actively dividing human cell line with freshly dissociated, highly differentiated cells from the mouse embryonic nervous system. The majority of the resulting hybrid strains retain human while losing mouse chromosomes and isozymes and thus provide a new class of hybrid cell for analysis of gene linkage and regulation.

AB - THE usual pattern of chromosome segregation in interspecific human×mouse somatic cell hybrids is retention of mouse and loss of human chromosomes1. In attempts to generate hybrid strains expressing differentiated functions, we have fused an actively dividing human cell line with freshly dissociated, highly differentiated cells from the mouse embryonic nervous system. The majority of the resulting hybrid strains retain human while losing mouse chromosomes and isozymes and thus provide a new class of hybrid cell for analysis of gene linkage and regulation.

UR - http://www.scopus.com/inward/record.url?scp=0016163588&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016163588&partnerID=8YFLogxK

U2 - 10.1038/252401a0

DO - 10.1038/252401a0

M3 - Article

VL - 252

SP - 401

EP - 404

JO - Nature

JF - Nature

SN - 0028-0836

IS - 5482

ER -