Human x mouse hybrid cells segregating mouse chromosomes and isozymes

John D. Minna; Hayden G. Coon

doi:10.1038/252401a0

Human x mouse hybrid cells segregating mouse chromosomes and isozymes

John D. Minna, Hayden G. Coon

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Abstract

THE usual pattern of chromosome segregation in interspecific human×mouse somatic cell hybrids is retention of mouse and loss of human chromosomes¹. In attempts to generate hybrid strains expressing differentiated functions, we have fused an actively dividing human cell line with freshly dissociated, highly differentiated cells from the mouse embryonic nervous system. The majority of the resulting hybrid strains retain human while losing mouse chromosomes and isozymes and thus provide a new class of hybrid cell for analysis of gene linkage and regulation.

Original language	English (US)
Pages (from-to)	401-404
Number of pages	4
Journal	Nature
Volume	252
Issue number	5482
DOIs	https://doi.org/10.1038/252401a0
State	Published - 1974

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title = "Human x mouse hybrid cells segregating mouse chromosomes and isozymes",

abstract = "THE usual pattern of chromosome segregation in interspecific human×mouse somatic cell hybrids is retention of mouse and loss of human chromosomes1. In attempts to generate hybrid strains expressing differentiated functions, we have fused an actively dividing human cell line with freshly dissociated, highly differentiated cells from the mouse embryonic nervous system. The majority of the resulting hybrid strains retain human while losing mouse chromosomes and isozymes and thus provide a new class of hybrid cell for analysis of gene linkage and regulation.",

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AB - THE usual pattern of chromosome segregation in interspecific human×mouse somatic cell hybrids is retention of mouse and loss of human chromosomes1. In attempts to generate hybrid strains expressing differentiated functions, we have fused an actively dividing human cell line with freshly dissociated, highly differentiated cells from the mouse embryonic nervous system. The majority of the resulting hybrid strains retain human while losing mouse chromosomes and isozymes and thus provide a new class of hybrid cell for analysis of gene linkage and regulation.

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Human x mouse hybrid cells segregating mouse chromosomes and isozymes

Abstract

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