Huntingtin interacting protein 1 is a clathrin coat binding protein required for differentiation of late spermatogenic progenitors

D. S. Rao, J. C. Chang, P. D. Kumar, I. Mizukami, G. M. Smithson, S. V. Bradley, A. F. Parlow, T. S. Ross

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Huntingtin-interacting protein 1 (HIP1) interacts with huntingtin, the protein whose gene is mutated in Huntington's disease. In addition, a fusion between HIP1 and platelet-derived growth factor β receptor causes chronic myelomonocytic leukemia. The HIP1 proteins, including HIP1 and HIP1-related (HIP1r), have an N-terminal polyphosphoinositide-interacting epsin N-terminal homology, domain, which is found in proteins involved in clathrin-mediated endocytosis. HIP1 and HIP1r also share a central leucine zipper and an actin binding TALIN homology domain. Here we show that HIP1, like HIP1r, colocalizes with clathrin coat components. We also show that HIP1 physically associates with clathrin and AP-2, the major components of the clathrin coat. To further understand the putative biological role(s) of HIP1, we have generated a targeted deletion of murine HIP1. HIP1-/- mice developed into adulthood, did not develop overt neurologic symptoms in the first year of life, and had normal peripheral blood counts. However, HIP1-deficient mice exhibited testicular degeneration with increased apoptosis of postmeiotic spermatids. Postmeiotic spermatids are the only cells of the seminiferous tubules that express HIP1. These findings indicate that HIP1 is required for differentiation, proliferation, and/or survival of spermatogenic progenitors. The association of HIP1 with clathrin coats and the requirement of HIP1 for progenitor survival suggest a role for HIP1 in the regulation of endocytosis.

Original languageEnglish (US)
Pages (from-to)7796-7806
Number of pages11
JournalMolecular and Cellular Biology
Volume21
Issue number22
DOIs
StatePublished - 2001

Fingerprint

Clathrin
Capsid Proteins
Carrier Proteins
Spermatids
Huntingtin Protein
Endocytosis
Leukemia, Myelomonocytic, Chronic
Platelet-Derived Growth Factor Receptors
Phosphatidylinositol Phosphates
Leucine Zippers
Seminiferous Tubules
Huntington Disease
Neurologic Manifestations

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Huntingtin interacting protein 1 is a clathrin coat binding protein required for differentiation of late spermatogenic progenitors. / Rao, D. S.; Chang, J. C.; Kumar, P. D.; Mizukami, I.; Smithson, G. M.; Bradley, S. V.; Parlow, A. F.; Ross, T. S.

In: Molecular and Cellular Biology, Vol. 21, No. 22, 2001, p. 7796-7806.

Research output: Contribution to journalArticle

Rao, D. S. ; Chang, J. C. ; Kumar, P. D. ; Mizukami, I. ; Smithson, G. M. ; Bradley, S. V. ; Parlow, A. F. ; Ross, T. S. / Huntingtin interacting protein 1 is a clathrin coat binding protein required for differentiation of late spermatogenic progenitors. In: Molecular and Cellular Biology. 2001 ; Vol. 21, No. 22. pp. 7796-7806.
@article{90cd9aea77444f6aaa0a939308cbb496,
title = "Huntingtin interacting protein 1 is a clathrin coat binding protein required for differentiation of late spermatogenic progenitors",
abstract = "Huntingtin-interacting protein 1 (HIP1) interacts with huntingtin, the protein whose gene is mutated in Huntington's disease. In addition, a fusion between HIP1 and platelet-derived growth factor β receptor causes chronic myelomonocytic leukemia. The HIP1 proteins, including HIP1 and HIP1-related (HIP1r), have an N-terminal polyphosphoinositide-interacting epsin N-terminal homology, domain, which is found in proteins involved in clathrin-mediated endocytosis. HIP1 and HIP1r also share a central leucine zipper and an actin binding TALIN homology domain. Here we show that HIP1, like HIP1r, colocalizes with clathrin coat components. We also show that HIP1 physically associates with clathrin and AP-2, the major components of the clathrin coat. To further understand the putative biological role(s) of HIP1, we have generated a targeted deletion of murine HIP1. HIP1-/- mice developed into adulthood, did not develop overt neurologic symptoms in the first year of life, and had normal peripheral blood counts. However, HIP1-deficient mice exhibited testicular degeneration with increased apoptosis of postmeiotic spermatids. Postmeiotic spermatids are the only cells of the seminiferous tubules that express HIP1. These findings indicate that HIP1 is required for differentiation, proliferation, and/or survival of spermatogenic progenitors. The association of HIP1 with clathrin coats and the requirement of HIP1 for progenitor survival suggest a role for HIP1 in the regulation of endocytosis.",
author = "Rao, {D. S.} and Chang, {J. C.} and Kumar, {P. D.} and I. Mizukami and Smithson, {G. M.} and Bradley, {S. V.} and Parlow, {A. F.} and Ross, {T. S.}",
year = "2001",
doi = "10.1128/MCB.21.22.7796-7806.2001",
language = "English (US)",
volume = "21",
pages = "7796--7806",
journal = "Molecular and Cellular Biology",
issn = "0270-7306",
publisher = "American Society for Microbiology",
number = "22",

}

TY - JOUR

T1 - Huntingtin interacting protein 1 is a clathrin coat binding protein required for differentiation of late spermatogenic progenitors

AU - Rao, D. S.

AU - Chang, J. C.

AU - Kumar, P. D.

AU - Mizukami, I.

AU - Smithson, G. M.

AU - Bradley, S. V.

AU - Parlow, A. F.

AU - Ross, T. S.

PY - 2001

Y1 - 2001

N2 - Huntingtin-interacting protein 1 (HIP1) interacts with huntingtin, the protein whose gene is mutated in Huntington's disease. In addition, a fusion between HIP1 and platelet-derived growth factor β receptor causes chronic myelomonocytic leukemia. The HIP1 proteins, including HIP1 and HIP1-related (HIP1r), have an N-terminal polyphosphoinositide-interacting epsin N-terminal homology, domain, which is found in proteins involved in clathrin-mediated endocytosis. HIP1 and HIP1r also share a central leucine zipper and an actin binding TALIN homology domain. Here we show that HIP1, like HIP1r, colocalizes with clathrin coat components. We also show that HIP1 physically associates with clathrin and AP-2, the major components of the clathrin coat. To further understand the putative biological role(s) of HIP1, we have generated a targeted deletion of murine HIP1. HIP1-/- mice developed into adulthood, did not develop overt neurologic symptoms in the first year of life, and had normal peripheral blood counts. However, HIP1-deficient mice exhibited testicular degeneration with increased apoptosis of postmeiotic spermatids. Postmeiotic spermatids are the only cells of the seminiferous tubules that express HIP1. These findings indicate that HIP1 is required for differentiation, proliferation, and/or survival of spermatogenic progenitors. The association of HIP1 with clathrin coats and the requirement of HIP1 for progenitor survival suggest a role for HIP1 in the regulation of endocytosis.

AB - Huntingtin-interacting protein 1 (HIP1) interacts with huntingtin, the protein whose gene is mutated in Huntington's disease. In addition, a fusion between HIP1 and platelet-derived growth factor β receptor causes chronic myelomonocytic leukemia. The HIP1 proteins, including HIP1 and HIP1-related (HIP1r), have an N-terminal polyphosphoinositide-interacting epsin N-terminal homology, domain, which is found in proteins involved in clathrin-mediated endocytosis. HIP1 and HIP1r also share a central leucine zipper and an actin binding TALIN homology domain. Here we show that HIP1, like HIP1r, colocalizes with clathrin coat components. We also show that HIP1 physically associates with clathrin and AP-2, the major components of the clathrin coat. To further understand the putative biological role(s) of HIP1, we have generated a targeted deletion of murine HIP1. HIP1-/- mice developed into adulthood, did not develop overt neurologic symptoms in the first year of life, and had normal peripheral blood counts. However, HIP1-deficient mice exhibited testicular degeneration with increased apoptosis of postmeiotic spermatids. Postmeiotic spermatids are the only cells of the seminiferous tubules that express HIP1. These findings indicate that HIP1 is required for differentiation, proliferation, and/or survival of spermatogenic progenitors. The association of HIP1 with clathrin coats and the requirement of HIP1 for progenitor survival suggest a role for HIP1 in the regulation of endocytosis.

UR - http://www.scopus.com/inward/record.url?scp=0034780505&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034780505&partnerID=8YFLogxK

U2 - 10.1128/MCB.21.22.7796-7806.2001

DO - 10.1128/MCB.21.22.7796-7806.2001

M3 - Article

C2 - 11604514

AN - SCOPUS:0034780505

VL - 21

SP - 7796

EP - 7806

JO - Molecular and Cellular Biology

JF - Molecular and Cellular Biology

SN - 0270-7306

IS - 22

ER -