TY - JOUR
T1 - Hybrid Nanofibrous Composites with Anisotropic Mechanics and Architecture for Tendon/Ligament Repair and Regeneration
AU - Li, Jun
AU - Xue, Chao
AU - Wang, Hao
AU - Dong, Shiyan
AU - Yang, Zhaogang
AU - Cao, Yuting
AU - Zhao, Binan
AU - Cheng, Biao
AU - Xie, Xianrui
AU - Mo, Xiumei
AU - Jiang, Wen
AU - Yuan, Hengfeng
AU - Pan, Jianfeng
N1 - Funding Information:
J.L., C.X., and H.W. contributed equally to this work. This study was supported by the National Nature Science Foundation of China (81802144, 81702133), the Research Project of Shanghai Municipal Health Commission (Contract Grant No. 20194Y0316), and the Excellent Youth Training Program of Shanghai Jiaotong University Affiliated Sixth People's Hospital (ynyq202102).
Publisher Copyright:
© 2022 Wiley-VCH GmbH.
PY - 2022/7/7
Y1 - 2022/7/7
N2 - Rupture of tendons and ligaments (T/L) is a major clinical challenge due to T/L possess anisotropic mechanical properties and hierarchical structures. Here, to imitate these characteristics, an approach is presented by fabricating hybrid nanofibrous composites. First, hybrid fiber-reinforced yarns are fabricated via successively electrospinning poly(L‑lactide‑co‑ε‑caprolactone) (PLCL) and gelatin (Ge) nanofibers onto polyethylene terephthalate (PET) fibers to improve biodurability and biocompatibility. Then, by comparing different manufacturing methods, the knitted structure succeeds in simulating anisotropic mechanical properties, even being stronger than natural ligaments, and possessing comfort compliance superior to clinically used ligament advanced reinforcement system (LARS) ligament. Moreover, after inoculation with tendon-derived stem cells and transplantation in vivo, hybrid nanofibrous composites are integrated with native tendons to guide surrounding tissue ingrowth due to the highly interconnected and porous structure. The knitted hybrid nanofibrous composites are also ligamentized and remodeled in vivo to promote tendon regeneration. Specifically, after the use of optimized anisotropic hybrid nanofibrous composites to repair tendon, the deposition of tendon-associated extracellular matrix proteins is more significant. Thus, this study indicates a strategy of manufacturing anisotropic hybrid nanofibrous composites with superior mechanical properties and good histocompatibility for clinical reconstruction.
AB - Rupture of tendons and ligaments (T/L) is a major clinical challenge due to T/L possess anisotropic mechanical properties and hierarchical structures. Here, to imitate these characteristics, an approach is presented by fabricating hybrid nanofibrous composites. First, hybrid fiber-reinforced yarns are fabricated via successively electrospinning poly(L‑lactide‑co‑ε‑caprolactone) (PLCL) and gelatin (Ge) nanofibers onto polyethylene terephthalate (PET) fibers to improve biodurability and biocompatibility. Then, by comparing different manufacturing methods, the knitted structure succeeds in simulating anisotropic mechanical properties, even being stronger than natural ligaments, and possessing comfort compliance superior to clinically used ligament advanced reinforcement system (LARS) ligament. Moreover, after inoculation with tendon-derived stem cells and transplantation in vivo, hybrid nanofibrous composites are integrated with native tendons to guide surrounding tissue ingrowth due to the highly interconnected and porous structure. The knitted hybrid nanofibrous composites are also ligamentized and remodeled in vivo to promote tendon regeneration. Specifically, after the use of optimized anisotropic hybrid nanofibrous composites to repair tendon, the deposition of tendon-associated extracellular matrix proteins is more significant. Thus, this study indicates a strategy of manufacturing anisotropic hybrid nanofibrous composites with superior mechanical properties and good histocompatibility for clinical reconstruction.
KW - anisotropic architecture
KW - biocompatibility
KW - mechanical durability
KW - nanofibrous composites
KW - tendon/ligament repair
UR - http://www.scopus.com/inward/record.url?scp=85131526126&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85131526126&partnerID=8YFLogxK
U2 - 10.1002/smll.202201147
DO - 10.1002/smll.202201147
M3 - Article
C2 - 35686342
AN - SCOPUS:85131526126
SN - 1613-6810
VL - 18
JO - Small
JF - Small
IS - 27
M1 - 2201147
ER -