Hyperoxia and self- or neutrophil-generated O2 metabolites inactivate xanthine oxidase

L. S. Terada, C. J. Beehler, A. Banerjee, J. M. Brown, M. A. Grosso, A. H. Harken, J. M. McCord, J. E. Repine

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Abstract

Xanthine oxidase (XO) and xanthine dehydrogenase (XD) activities decreased in lungs isolated from rats and cultured lung endothelial cells that had been exposed to hyperoxia. Purified XO activity also decreased after addition of a variety of chemically generated O2 metabolite species (superoxide anion, hydrogen peroxide, hydroxyl radical, or hypochlorous acid), hypoxanthine, or stimulated neutrophils in vitro. XO inactivation by chemically, self-, or neutrophil-generated O2 metabolites was decreased by simultaneous addition of various O2 metabolite scavengers but not their inactive analogues. Since XO appears to contribute to a variety of biological processes and diseases, hyperoxia- or O2 metabolite-mediated decreases in XO activity may be an important cellular control mechanism.

Original languageEnglish (US)
Pages (from-to)2349-2353
Number of pages5
JournalJournal of Applied Physiology
Volume65
Issue number5
Publication statusPublished - 1988

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ASJC Scopus subject areas

  • Endocrinology
  • Physiology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Terada, L. S., Beehler, C. J., Banerjee, A., Brown, J. M., Grosso, M. A., Harken, A. H., ... Repine, J. E. (1988). Hyperoxia and self- or neutrophil-generated O2 metabolites inactivate xanthine oxidase. Journal of Applied Physiology, 65(5), 2349-2353.