Hyperoxia and self- or neutrophil-generated O2 metabolites inactivate xanthine oxidase

L. S. Terada; C. J. Beehler; A. Banerjee; J. M. Brown; M. A. Grosso; A. H. Harken; J. M. McCord; J. E. Repine

doi:10.1152/jappl.1988.65.5.2349

Hyperoxia and self- or neutrophil-generated O₂ metabolites inactivate xanthine oxidase

L. S. Terada, C. J. Beehler, A. Banerjee, J. M. Brown, M. A. Grosso, A. H. Harken, J. M. McCord, J. E. Repine

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48 Scopus citations

Abstract

Xanthine oxidase (XO) and xanthine dehydrogenase (XD) activities decreased in lungs isolated from rats and cultured lung endothelial cells that had been exposed to hyperoxia. Purified XO activity also decreased after addition of a variety of chemically generated O₂ metabolite species (superoxide anion, hydrogen peroxide, hydroxyl radical, or hypochlorous acid), hypoxanthine, or stimulated neutrophils in vitro. XO inactivation by chemically, self-, or neutrophil-generated O₂ metabolites was decreased by simultaneous addition of various O₂ metabolite scavengers but not their inactive analogues. Since XO appears to contribute to a variety of biological processes and diseases, hyperoxia- or O₂ metabolite-mediated decreases in XO activity may be an important cellular control mechanism.

Original language	English (US)
Pages (from-to)	2349-2353
Number of pages	5
Journal	Journal of applied physiology
Volume	65
Issue number	5
DOIs	https://doi.org/10.1152/jappl.1988.65.5.2349
State	Published - 1988

ASJC Scopus subject areas

Physiology
Physiology (medical)

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10.1152/jappl.1988.65.5.2349

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title = "Hyperoxia and self- or neutrophil-generated O2 metabolites inactivate xanthine oxidase",

abstract = "Xanthine oxidase (XO) and xanthine dehydrogenase (XD) activities decreased in lungs isolated from rats and cultured lung endothelial cells that had been exposed to hyperoxia. Purified XO activity also decreased after addition of a variety of chemically generated O2 metabolite species (superoxide anion, hydrogen peroxide, hydroxyl radical, or hypochlorous acid), hypoxanthine, or stimulated neutrophils in vitro. XO inactivation by chemically, self-, or neutrophil-generated O2 metabolites was decreased by simultaneous addition of various O2 metabolite scavengers but not their inactive analogues. Since XO appears to contribute to a variety of biological processes and diseases, hyperoxia- or O2 metabolite-mediated decreases in XO activity may be an important cellular control mechanism.",

author = "Terada, {L. S.} and Beehler, {C. J.} and A. Banerjee and Brown, {J. M.} and Grosso, {M. A.} and Harken, {A. H.} and McCord, {J. M.} and Repine, {J. E.}",

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T1 - Hyperoxia and self- or neutrophil-generated O2 metabolites inactivate xanthine oxidase

AU - Terada, L. S.

AU - Beehler, C. J.

AU - Banerjee, A.

AU - Brown, J. M.

AU - Grosso, M. A.

AU - Harken, A. H.

AU - McCord, J. M.

AU - Repine, J. E.

PY - 1988

Y1 - 1988

N2 - Xanthine oxidase (XO) and xanthine dehydrogenase (XD) activities decreased in lungs isolated from rats and cultured lung endothelial cells that had been exposed to hyperoxia. Purified XO activity also decreased after addition of a variety of chemically generated O2 metabolite species (superoxide anion, hydrogen peroxide, hydroxyl radical, or hypochlorous acid), hypoxanthine, or stimulated neutrophils in vitro. XO inactivation by chemically, self-, or neutrophil-generated O2 metabolites was decreased by simultaneous addition of various O2 metabolite scavengers but not their inactive analogues. Since XO appears to contribute to a variety of biological processes and diseases, hyperoxia- or O2 metabolite-mediated decreases in XO activity may be an important cellular control mechanism.

AB - Xanthine oxidase (XO) and xanthine dehydrogenase (XD) activities decreased in lungs isolated from rats and cultured lung endothelial cells that had been exposed to hyperoxia. Purified XO activity also decreased after addition of a variety of chemically generated O2 metabolite species (superoxide anion, hydrogen peroxide, hydroxyl radical, or hypochlorous acid), hypoxanthine, or stimulated neutrophils in vitro. XO inactivation by chemically, self-, or neutrophil-generated O2 metabolites was decreased by simultaneous addition of various O2 metabolite scavengers but not their inactive analogues. Since XO appears to contribute to a variety of biological processes and diseases, hyperoxia- or O2 metabolite-mediated decreases in XO activity may be an important cellular control mechanism.

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Hyperoxia and self- or neutrophil-generated O₂ metabolites inactivate xanthine oxidase

Abstract

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