Hypoxic neuronal injury in vitro depends on extracellular glutamine

Mark P. Goldberg, Hannelore Monyer, Dennis W. Choi

Research output: Contribution to journalArticle

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Abstract

Hypoxic neuronal injury (HNI) in cortical cell cultures was enhanced in a concentration-dependent fashion by the presence of 500 μM to 2 mM (EC50 about 500 μM) glutamine in the medium, concentrations approximating those normally present in cerebrospinal fluid (CSF). Regardless of the glutamine concentration, glutamate receptor antagonists 2-amino-5-phosphonovalerate or dextrorphan could substantially reduce HNI. Thus, the availability of extracellular glutamine could be a determinant of hypoxic neuronal injury in vivo, most likely reflecting its importance in the synthesis of the neurotransmitter excitotoxins glutamate and aspartate.

Original languageEnglish (US)
Pages (from-to)52-57
Number of pages6
JournalNeuroscience Letters
Volume94
Issue number1-2
DOIs
StatePublished - Nov 22 1988

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Keywords

  • Aspartate
  • Cell culture
  • Glutamate
  • Glutamine
  • Hypoxia
  • Neurotoxicity

ASJC Scopus subject areas

  • Neuroscience(all)

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