IκB overexpression in cardiomyocytes prevents NF-κB translocation and provides cardioprotection in trauma

Deborah L. Carlson, D. Jean White, David L. Maass, Robin C. Nguyen, Brett Giroir, Jureta W. Horton

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

This study examined the effects of either IκBα overexpression (transgenic mice) or N-acetyl-leucinyl-leucinyl-norleucinal (ALLN) administration (proteosome inhibitor in wild-type mice) on cardiomyocyte secretion of tumor necrosis factor-α (TNF-α) and on cardiac performance after burn trauma. Transgenic mice were divided into four experimental groups. IκBα overexpressing mice were given a third-degree scald burn over 40% of the total body surface area or wild-type littermates were given either a scald or sham burn to provide appropriate controls. Pharmacological studies included ALLN (20 mg/kg) administration in either burned wild-type mice or wild-type shams. Burn trauma in wild-type mice promoted nuclear factor-κB (NF-κB) nuclear translocation, cardiomyocyte secretion of TNF-α, and impaired cardiac performance. IκBα overexpression or ALLN treatment of burn trauma prevented NF-κB activation in cardiac tissue, prevented cardiomyocyte secretion of TNF-α, and ablated burn-mediated cardiac contractile dysfunction. These data suggest that NF-κB activation and inflammatory cytokine secretion play a significant role in post-burn myocardial abnormalities.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume284
Issue number3 53-3
StatePublished - Mar 1 2003

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Cardiac Myocytes
Tumor Necrosis Factor-alpha
Wounds and Injuries
Burns
Transgenic Mice
Body Surface Area
Pharmacology
Cytokines

Keywords

  • Cardiac contractile function
  • Collagenase digestion
  • Fura 2-AM
  • N-acetyl-leucinyl-leucinyl-norleucinal

ASJC Scopus subject areas

  • Physiology

Cite this

IκB overexpression in cardiomyocytes prevents NF-κB translocation and provides cardioprotection in trauma. / Carlson, Deborah L.; White, D. Jean; Maass, David L.; Nguyen, Robin C.; Giroir, Brett; Horton, Jureta W.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 284, No. 3 53-3, 01.03.2003.

Research output: Contribution to journalArticle

Carlson, Deborah L. ; White, D. Jean ; Maass, David L. ; Nguyen, Robin C. ; Giroir, Brett ; Horton, Jureta W. / IκB overexpression in cardiomyocytes prevents NF-κB translocation and provides cardioprotection in trauma. In: American Journal of Physiology - Heart and Circulatory Physiology. 2003 ; Vol. 284, No. 3 53-3.
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