Identification and assay of allosteric regulators of S-adenosylmethionine decarboxylase.

Erin K. Willert, Lisa N. Kinch, Margaret A. Phillips

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Polyamine biosynthesis is extensively regulated in cells by multiple mechanisms, including regulation of enzyme activity posttranslationally. The identified regulatory factors include both small molecules and regulatory proteins, and the mechanisms vary in different species across the evolutionary tree. Based on this diversity of mechanism, it is likely that regulatory factors of the pathway remain unidentified in many species. This article focuses on methods for identifying novel regulatory factors of polyamine biosynthesis as illustrated by the discovery of a novel protein activator of the key biosynthetic enzyme S-adenosylmethionine decarboxylase in the protozoan trypanosomatid parasites.

Original languageEnglish (US)
Pages (from-to)219-235
Number of pages17
JournalMethods in molecular biology (Clifton, N.J.)
Volume720
DOIs
StatePublished - May 24 2011

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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