Identification and biosynthesis of cyclic enterobacterial common antigen in Escherichia coli

Paul J A Erbel, Kathleen Barr, Ninguo Gao, Gerrit J. Gerwig, Paul D. Rick, Kevin H. Gardner

Research output: Contribution to journalArticle

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Abstract

Phosphoglyceride-linked enterobacterial common antigen (ECAPG) is a cell surface glycolipid that is synthesized by all gram-negative enteric bacteria. The carbohydrate portion of ECAPG consists of linear heteropolysaccharide chains comprised of the trisaccharide repeat unit Fuc4NAc-ManNAcA-GlcNAc, where Fuc4NAc is 4-acetamido-4,6-dideoxy-D-galactose, ManNAcA is N-acetyl-D-mannosaminuronic acid, and GlcNAc is N-acetyl-D-glucosamine. The potential reducing terminal GlcNAc residue of each polysaccharide chain is linked via phosphodiester linkage to a phosphoglyceride aglycone. We demonstrate here the occurrence of a watersoluble cyclic form of enterobacterial common antigen, ECACYC, purified from Escherichia coli strains B and K-12 with solution nuclear magnetic resonance (NMR) spectroscopy, electrospray ionization mass spectrometry (ESI-MS), and additional biochemical methods. The ECACYC molecules lacked an aglycone and contained four trisaccharide repeat units that were nonstoichiometrically substituted with up to four O-acetyl groups. ECACYC was not detected in mutant strains that possessed null mutations in the wecA, wecF, and wecG genes of the wec gene cluster. These observations corroborate the structural data obtained by NMR and ESI-MS analyses and show for the first time that the trisaccharide repeat units of ECACYC and ECAPG are assembled by a common biosynthetic pathway.

Original languageEnglish (US)
Pages (from-to)1995-2004
Number of pages10
JournalJournal of Bacteriology
Volume185
Issue number6
DOIs
StatePublished - Mar 2003

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Trisaccharides
Glycerophospholipids
Electrospray Ionization Mass Spectrometry
Escherichia coli
Magnetic Resonance Spectroscopy
Acetylglucosamine
Glycolipids
Biosynthetic Pathways
Enterobacteriaceae
Multigene Family
Gram-Negative Bacteria
Galactose
Polysaccharides
Carbohydrates
Mutation
Genes
enterobacterial common antigen
4-acetamido-4,6-dideoxygalactose

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Identification and biosynthesis of cyclic enterobacterial common antigen in Escherichia coli. / Erbel, Paul J A; Barr, Kathleen; Gao, Ninguo; Gerwig, Gerrit J.; Rick, Paul D.; Gardner, Kevin H.

In: Journal of Bacteriology, Vol. 185, No. 6, 03.2003, p. 1995-2004.

Research output: Contribution to journalArticle

Erbel, Paul J A ; Barr, Kathleen ; Gao, Ninguo ; Gerwig, Gerrit J. ; Rick, Paul D. ; Gardner, Kevin H. / Identification and biosynthesis of cyclic enterobacterial common antigen in Escherichia coli. In: Journal of Bacteriology. 2003 ; Vol. 185, No. 6. pp. 1995-2004.
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