Identification and characterization of a suite of tumor targeting peptides for non-small cell lung cancer

Michael J. McGuire, Bethany Powell Gray, Shunzi Li, Dorothy Cupka, Lauren Averett Byers, Lei Wu, Shaghayegh Rezaie, Ying Horng Liu, Naveen Pattisapu, James Issac, Tsukasa Oyama, Lixia Diao, John V. Heymach, Xian Jin Xie, John D. Minna, Kathlynn C. Brown

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36 Scopus citations

Abstract

Tumor targeting ligands are emerging components in cancer therapies. Widespread use of targeted therapies and molecular imaging is dependent on increasing the number of high affinity, tumor-specific ligands. Towards this goal, we biopanned three phage-displayed peptide libraries on a series of well-defined human non-small cell lung cancer (NSCLC) cell lines, isolating 11 novel peptides. The peptides show distinct binding profiles across 40 NSCLC cell lines and do not bind normal bronchial epithelial cell lines. Binding of specific peptides correlates with onco-genotypes and activation of particular pathways, such as EGFR signaling, suggesting the peptides may serve as surrogate markers. Multimerization of the peptides results in cell binding affinities between 0.0071-40â.nM. The peptides home to tumors in vivo and bind to patient tumor samples. This is the first comprehensive biopanning for isolation of high affinity peptidic ligands for a single cancer type and expands the diversity of NSCLC targeting ligands.

Original languageEnglish (US)
Article number4480
JournalScientific reports
Volume4
DOIs
StatePublished - Mar 27 2014

ASJC Scopus subject areas

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    McGuire, M. J., Gray, B. P., Li, S., Cupka, D., Byers, L. A., Wu, L., Rezaie, S., Liu, Y. H., Pattisapu, N., Issac, J., Oyama, T., Diao, L., Heymach, J. V., Xie, X. J., Minna, J. D., & Brown, K. C. (2014). Identification and characterization of a suite of tumor targeting peptides for non-small cell lung cancer. Scientific reports, 4, [4480]. https://doi.org/10.1038/srep04480