Identification and characterization of cellular heterogeneity within the developing renal interstitium

Alicia R. England, Christopher Chaney, Amrita Das, Mohita Patel, Alicia Malewska, Daniel Armendariz, Gary Hon, Douglas W. Strand, Keri A. Drake, Thomas J. Carroll

Research output: Contribution to journalArticle

Abstract

Kidney formation requires the coordinated growth of multiple cell types including the collecting ducts, nephrons, vasculature and interstitium. There is a long-held belief that interactions between progenitors of the collecting ducts and nephrons are primarily responsible for kidney development. However, over the last several years, it has become increasingly clear that multiple aspects of kidney development require signaling from the interstitium. How the interstitium orchestrates these various roles is poorly understood. Here, we show that during development the interstitium is a highly heterogeneous patterned population of cells that occupies distinct positions correlated to the adjacent parenchyma. Our analysis indicates that the heterogeneity is not a mere reflection of different stages in a linear developmental trajectory but instead represents several novel differentiated cell states. Further, we find that β-catenin has a cell autonomous role in the development of a medullary subset of the interstitium and that this non-autonomously affects the development of the adjacent epithelia. These findings suggest the intriguing possibility that the different interstitial subtypes may create microenvironments that play unique roles in development of the adjacent epithelia and endothelia.

Original languageEnglish (US)
JournalDevelopment (Cambridge, England)
Volume147
Issue number15
DOIs
StatePublished - Aug 14 2020

Keywords

  • Fibroblast heterogeneity
  • Microenvironment
  • Patterned interstitium
  • Patterned stroma
  • Renal stroma

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

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