Identification and characterization of nonapeptide targeting a human B cell lymphoma, Raji

Jin Huk Choi, Woon Kyu Lee, Seung Hyun Han, Seckho Ha, Sung Min Ahn, Jae Seong Kang, Yun Jaie Choi, Cheol Heui Yun

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Here, we identified a novel peptide specifically targeting a human B cell lymphoma, Raji, through a conventional phage display method. The amino acid sequence, 'CTLPHLKMC' was obtained with the highest frequency from a nonapeptide-expressing phage library. The phage clone encoding CTLPHLKMC peptide sequence avidly bound to Raji cells compared with control phage clones. Furthermore, flow-cytometric analysis on the biotinylated synthetic CTLPHLKMC peptide demonstrated the high binding affinity to Raji cells in a dose-dependent manner whereas it has binding activity to neither human peripheral blood mononuclear cells including normal B cell derived from healthy donors nor other leukemia cells including THP-1, HL-60, Jurkat and IM-9. MALDI-TOF mass spectrometry following immunoprecipitation assay showed that a potential host receptor for the peptide is a variable region of human immunoglobulin heavy chain which would be a specific phenotypic marker of Raji. In conclusion, these results suggest that the peptide, 'CTLPHLKMC', is a specific ligand to a Raji cell.

Original languageEnglish (US)
Pages (from-to)852-858
Number of pages7
JournalInternational Immunopharmacology
Volume8
Issue number6
DOIs
StatePublished - Jun 1 2008
Externally publishedYes

Keywords

  • B cell lymphoma
  • Phage display
  • Raji
  • Targeting peptide

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Pharmacology

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