Identification and characterization of rat intestinal trefoil factor: Tissue- and cell-specific member of the trefoil protein family

S. Suemori, K. Lynch-Devaney, D. K. Podolsky

Research output: Contribution to journalArticlepeer-review

266 Scopus citations

Abstract

The trefoil peptide family encompasses a group of small proteins that appear to assume a distinctive secondary structure that leads to intrinsic resistance to protease digestion. Induction of these peptides has been associated with response to injury in the gastrointestinal tract and related organs. Using an oligonucleotide derived from N-terminal ammo acid sequencing of a transformed growth-inhibiting protein, a cDNA was cloned from rat intestinal villus epithelial cells that encodes a protein 81 amino acids in length with the characteristic trefoil peptide cysteine residue motif. Northern blot analysis demonstrates specific expression of a single transcript of 0.43 kilobase in small and large intestinal epithelium in rat and man. Indirect immunofluorescent staining with antiserum raised using a synthetic peptide based on the predicted C-terminal sequence of this protein, designated intestinal trefoil factor, demonstrated that it is primarily expressed and secreted onto the intestinal surface by goblet cells, suggesting that it may be an important component of intrinsic mechanisms for defending mucosal integrity.

Original languageEnglish (US)
Pages (from-to)11017-11021
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number24
DOIs
StatePublished - 1991

Keywords

  • Goblet cell
  • Growth factor
  • Intestine

ASJC Scopus subject areas

  • General

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