Identification and confirmation of biomarkers using an integrated platform for quantitative analysis of glycoproteins and their glycosylations

Yashu Liu, Jintang He, Chen Li, Ricardo Benitez, Sherry Fu, Jorge Marrero, David M. Lubman

Research output: Contribution to journalArticle

54 Scopus citations

Abstract

Hepatocellular carcinoma (HCC) is the most common primary malignant tumor of the liver. However, accurate diagnosis can be difficult as most of the patients who develop this tumor have symptoms similar to those caused by longstanding liver disease. Herein we developed an integrated platform to discover the glycoprotein biomarkers in early HCC. At first, lectin arrays were applied to investigate the differences in glycan structures on serum glycoproteins from HCC and cirrhosis patients. The intensity for AAL and LCA was significantly higher in HCC, indicating an elevation of fucosylation level. Then serum from 10 HCC samples and 10 cirrhosis samples were used to screen the altered fucosylated proteins by a combination of Exactag labeling, lectin extraction and LC-MS/MS. Finally, 27 HCC and 27 cirrhosis serum samples were used for lectin-antibody arrays to confirm the change of these fucosylated proteins. C3, CE, HRG, CD14 and HGF were found to be biomarker candidates for distinguishing early HCC from cirrhosis, with a sensitivity of 72% and specificity of 79%. Our work gives insight to the detection of early HCC, and the application of this comprehensive strategy has the potential to facilitate biomarker discovery on a large scale.

Original languageEnglish (US)
Pages (from-to)798-805
Number of pages8
JournalJournal of Proteome Research
Volume9
Issue number2
DOIs
StatePublished - Feb 5 2010

Keywords

  • Biomarkers
  • Cancer
  • Glycoproteins
  • Hepatocellular
  • Lectin arrays
  • Mass spectrometry

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Fingerprint Dive into the research topics of 'Identification and confirmation of biomarkers using an integrated platform for quantitative analysis of glycoproteins and their glycosylations'. Together they form a unique fingerprint.

  • Cite this