Identification and function of conformational dynamics in the multidomain GTPase dynamin

Saipraveen Srinivasan, Venkatasubramanian Dharmarajan, Dana Kim Reed, Patrick R. Griffin, Sandra L. Schmid

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Vesicle release upon endocytosis requires membrane fission, catalyzed by the large GTPase dynamin. Dynamin contains five domains that together orchestrate its mechanochemical activity. Hydrogen-deuterium exchange coupled with mass spectrometry revealed global nucleotide- and membrane-binding-dependent conformational changes, as well as the existence of an allosteric relay element in the α2S helix of the dynamin stalk domain. As predicted from structural studies, FRET analyses detect large movements of the pleckstrin homology domain (PHD) from a 'closed' conformation docked near the stalk to an 'open' conformation able to interact with membranes. We engineered dynamin constructs locked in either the closed or open state by chemical cross-linking or deletion mutagenesis and showed that PHD movements function as a conformational switch to regulate dynamin self-assembly, membrane binding, and fission. This PHD conformational switch is impaired by a centronuclear myopathy-causing disease mutation, S619L, highlighting the physiological significance of its role in regulating dynamin function. Together, these data provide new insight into coordinated conformational changes that regulate dynamin function and couple membrane binding, oligomerization, and GTPase activity during dynamin-catalyzed membrane fission.

Original languageEnglish (US)
Pages (from-to)443-457
Number of pages15
JournalEMBO Journal
Volume35
Issue number4
DOIs
StatePublished - Feb 15 2016

Fingerprint

Dynamins
GTP Phosphohydrolases
Membranes
Conformations
Congenital Structural Myopathies
Switches
Oligomerization
Mutagenesis
Deuterium
Endocytosis
Self assembly
Mass spectrometry
Hydrogen
Mass Spectrometry
Nucleotides
Mutation

Keywords

  • centronuclear myopathy
  • clathrin-mediated endocytosis
  • hydrogen-deuterium exchange
  • membrane fission
  • pleckstrin homology domain

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)

Cite this

Identification and function of conformational dynamics in the multidomain GTPase dynamin. / Srinivasan, Saipraveen; Dharmarajan, Venkatasubramanian; Reed, Dana Kim; Griffin, Patrick R.; Schmid, Sandra L.

In: EMBO Journal, Vol. 35, No. 4, 15.02.2016, p. 443-457.

Research output: Contribution to journalArticle

Srinivasan, Saipraveen ; Dharmarajan, Venkatasubramanian ; Reed, Dana Kim ; Griffin, Patrick R. ; Schmid, Sandra L. / Identification and function of conformational dynamics in the multidomain GTPase dynamin. In: EMBO Journal. 2016 ; Vol. 35, No. 4. pp. 443-457.
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