Identification and gene expression of bovine C-type lectin dectin-2

M. Bonkobara, M. Hoshino, H. Yagihara, K. Tamura, M. Isotani, Y. Tanaka, T. Washizu, K. Ariizumi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The C-type lectin receptor has been shown to recognize carbohydrate moieties of self and non-self antigens, thus serving as an innate immune receptor. Using bioinformatics and molecular cloning techniques, we isolated a bovine gene that encodes a polypeptide of 206 amino acids with structural features shared by mouse and human dectin-2, including a high homology with mouse dectin-2 (66%), a type II configuration, a short cytoplasmic domain without tyrosine-based signal motifs, a carbohydrate recognition domain, a putative N-glycosylation site, and an EPN motif involved in the Ca 2+-dependent binding of hexose carbohydrates. These results reveal this bovine gene to be a counterpart of mouse dectin-2. Moreover, the bovine dectin-2 gene showed heterogeneity in mRNA (the generation of alternatively spliced transcript) and segmentation into six exons, which are also observed in mouse dectin-2. Inconsistent with mouse dectin-2 mRNA, the bovine counterpart is abundantly expressed by Langerhans cells compared to macrophages; however, lymph nodes showed the highest expression level of bovine dectin-2, while spleen and lung showed the highest expression levels of mouse and human dectin-2. In cattle, dectin-2 expressed by dendritic cells may be clinically involved in the recognition of invading antigens in lymph nodes.

Original languageEnglish (US)
Pages (from-to)179-186
Number of pages8
JournalVeterinary Immunology and Immunopathology
Volume110
Issue number1-2
DOIs
StatePublished - Mar 15 2006

Fingerprint

C-Type Lectins
lectins
Gene Expression
gene expression
cattle
mice
carbohydrates
lymph nodes
Carbohydrates
Langerhans cells
antigens
genes
alternative splicing
hexoses
dendritic cells
glycosylation
Lymph Nodes
bioinformatics
Genes
exons

Keywords

  • Bovine
  • C-type lectin receptor
  • Cloning
  • Dectin-2
  • Langerhans/dendritic cell

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Immunology
  • veterinary(all)

Cite this

Identification and gene expression of bovine C-type lectin dectin-2. / Bonkobara, M.; Hoshino, M.; Yagihara, H.; Tamura, K.; Isotani, M.; Tanaka, Y.; Washizu, T.; Ariizumi, K.

In: Veterinary Immunology and Immunopathology, Vol. 110, No. 1-2, 15.03.2006, p. 179-186.

Research output: Contribution to journalArticle

Bonkobara, M, Hoshino, M, Yagihara, H, Tamura, K, Isotani, M, Tanaka, Y, Washizu, T & Ariizumi, K 2006, 'Identification and gene expression of bovine C-type lectin dectin-2', Veterinary Immunology and Immunopathology, vol. 110, no. 1-2, pp. 179-186. https://doi.org/10.1016/j.vetimm.2005.08.031
Bonkobara M, Hoshino M, Yagihara H, Tamura K, Isotani M, Tanaka Y et al. Identification and gene expression of bovine C-type lectin dectin-2. Veterinary Immunology and Immunopathology. 2006 Mar 15;110(1-2):179-186. https://doi.org/10.1016/j.vetimm.2005.08.031
Bonkobara, M. ; Hoshino, M. ; Yagihara, H. ; Tamura, K. ; Isotani, M. ; Tanaka, Y. ; Washizu, T. ; Ariizumi, K. / Identification and gene expression of bovine C-type lectin dectin-2. In: Veterinary Immunology and Immunopathology. 2006 ; Vol. 110, No. 1-2. pp. 179-186.
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