Identification and Partial Purification of a Factor That Stimulates Calcium-Dependent Proteases

George N. DeMartino; Donald K. Blumenthal

doi:10.1021/bi00261a019

Identification and Partial Purification of a Factor That Stimulates Calcium-Dependent Proteases

George N. DeMartino, Donald K. Blumenthal

Research output: Contribution to journal › Article › peer-review

65 Scopus citations

Abstract

We have identified and partially purified from bovine brain a factor which stimulates the activities of two rat liver calcium-dependent proteases. The factor, which is physically and functionally distinct from calmodulin, is heat stable and has a low apparent molecular weight (Mr 20000). The factor stimulates rates of proteolysis up to 25-fold but does not alter the calcium sensitivity of either protease. We have called this factor calcium-dependent protease regulator.

Original language	English (US)
Pages (from-to)	4297-4303
Number of pages	7
Journal	Biochemistry
Volume	21
Issue number	18
DOIs	https://doi.org/10.1021/bi00261a019
State	Published - Aug 1982

ASJC Scopus subject areas

Biochemistry

Access to Document

10.1021/bi00261a019

Cite this

@article{7119b71753424f2bb0e7a818853094e1,

title = "Identification and Partial Purification of a Factor That Stimulates Calcium-Dependent Proteases",

abstract = "We have identified and partially purified from bovine brain a factor which stimulates the activities of two rat liver calcium-dependent proteases. The factor, which is physically and functionally distinct from calmodulin, is heat stable and has a low apparent molecular weight (Mr 20000). The factor stimulates rates of proteolysis up to 25-fold but does not alter the calcium sensitivity of either protease. We have called this factor calcium-dependent protease regulator.",

author = "DeMartino, {George N.} and Blumenthal, {Donald K.}",

year = "1982",

month = aug,

doi = "10.1021/bi00261a019",

language = "English (US)",

volume = "21",

pages = "4297--4303",

journal = "Biochemistry",

issn = "0006-2960",

publisher = "American Chemical Society",

number = "18",

}

TY - JOUR

T1 - Identification and Partial Purification of a Factor That Stimulates Calcium-Dependent Proteases

AU - DeMartino, George N.

AU - Blumenthal, Donald K.

PY - 1982/8

Y1 - 1982/8

N2 - We have identified and partially purified from bovine brain a factor which stimulates the activities of two rat liver calcium-dependent proteases. The factor, which is physically and functionally distinct from calmodulin, is heat stable and has a low apparent molecular weight (Mr 20000). The factor stimulates rates of proteolysis up to 25-fold but does not alter the calcium sensitivity of either protease. We have called this factor calcium-dependent protease regulator.

AB - We have identified and partially purified from bovine brain a factor which stimulates the activities of two rat liver calcium-dependent proteases. The factor, which is physically and functionally distinct from calmodulin, is heat stable and has a low apparent molecular weight (Mr 20000). The factor stimulates rates of proteolysis up to 25-fold but does not alter the calcium sensitivity of either protease. We have called this factor calcium-dependent protease regulator.

UR - http://www.scopus.com/inward/record.url?scp=0020456150&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020456150&partnerID=8YFLogxK

U2 - 10.1021/bi00261a019

DO - 10.1021/bi00261a019

M3 - Article

C2 - 6289877

AN - SCOPUS:0020456150

SN - 0006-2960

VL - 21

SP - 4297

EP - 4303

JO - Biochemistry

JF - Biochemistry

IS - 18

ER -

Identification and Partial Purification of a Factor That Stimulates Calcium-Dependent Proteases

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this