Identification and two-photon imaging of oligodendrocyte in CA1 region of hippocampal slices

Wei Zhou, Woo Ping Ge, Shaoqun Zeng, Shumin Duan, Qingming Luo

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Oligodendrocyte (OL) plays a critical role in myelination and axon maintenance in central nervous system. Recent studies show that OL can also express NMDA receptors in development and pathological situations in white matter. There is still lack of studies about OL properties and function in gray matter of brain. Here we reported that some glial cells in CA1 region of rat hippocampal slices (P15-23) had distinct electrophysiological characteristics from the other glia cells in this region, while they displayed uniform properties with OL from white matter in previous report; therefore, they were considered as OL in hippocampus. By loading dye in recording pipette and imaging with two-photon laser scanning microscopy, we acquired the high spatial resolution, three-dimension images of these special cells in live slices. The OL in hippocampus shows a complex process-bearing shape and the distribution of several processes is parallel to Schaffer fiber in CA1 region. When stimulating Schaffer fiber, OL displays a long duration depolarization mediated by inward rectifier potassium channel. This suggested that the OL in CA1 region could sense the neuronal activity and contribute to potassium clearance.

Original languageEnglish (US)
Pages (from-to)598-602
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume352
Issue number3
DOIs
StatePublished - Jan 19 2007

Keywords

  • CA1 region
  • Hippocampal slice
  • Oligodendrocyte
  • Two-photon laser scanning microscopy
  • Whole-cell recording

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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