Identification of a cullin homology region in a subunit of the anaphase- promoting complex

Hongtao Yu, Jan Michael Peters, Randall W. King, Andrew M. Page, Philip Hieter, Marc W. Kirschner

Research output: Contribution to journalArticlepeer-review

200 Scopus citations

Abstract

The anaphase-promoting complex is composed of eight protein subunits, including BimE (APC1), CDC27 (APC3), CDC16 (APC6), and CDC23 (APC8). The remaining four human APC subunits, APC2, APC4, APC5, and APC7, as well as human CDC23, were cloned. APC7 contains multiple copies of the tetratico peptide repeat, similar to CDC16, CDC23, and CDC27. Whereas APC4 and APC5 share no similarity to proteins of known function, APC2 contains a region that is similar to a sequence in cullins, a family of proteins implicated in the ubiquitination of G1 phase cyclins and cyclin-dependent kinase inhibitors. The APC2 gene is essential in Saccharomyces cerevisiae, and apc2 mutants arrest at metaphase and are defective in the degradation of Pds1p. APC2 and cullins may be distantly related members of a ubiquitin ligase family that targets cell cycle regulators for degradation.

Original languageEnglish (US)
Pages (from-to)1219-1222
Number of pages4
JournalScience
Volume279
Issue number5354
DOIs
StatePublished - Feb 20 1998

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Identification of a cullin homology region in a subunit of the anaphase- promoting complex'. Together they form a unique fingerprint.

Cite this