Identification of a cullin homology region in a subunit of the anaphase- promoting complex

Hongtao Yu, Jan Michael Peters, Randall W. King, Andrew M. Page, Philip Hieter, Marc W. Kirschner

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Abstract

The anaphase-promoting complex is composed of eight protein subunits, including BimE (APC1), CDC27 (APC3), CDC16 (APC6), and CDC23 (APC8). The remaining four human APC subunits, APC2, APC4, APC5, and APC7, as well as human CDC23, were cloned. APC7 contains multiple copies of the tetratico peptide repeat, similar to CDC16, CDC23, and CDC27. Whereas APC4 and APC5 share no similarity to proteins of known function, APC2 contains a region that is similar to a sequence in cullins, a family of proteins implicated in the ubiquitination of G1 phase cyclins and cyclin-dependent kinase inhibitors. The APC2 gene is essential in Saccharomyces cerevisiae, and apc2 mutants arrest at metaphase and are defective in the degradation of Pds1p. APC2 and cullins may be distantly related members of a ubiquitin ligase family that targets cell cycle regulators for degradation.

Original languageEnglish (US)
Pages (from-to)1219-1222
Number of pages4
JournalScience
Volume279
Issue number5354
DOIs
StatePublished - Feb 20 1998

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Cullin Proteins
Anaphase-Promoting Complex-Cyclosome
Cyclins
Cyclin-Dependent Kinases
Ubiquitination
Essential Genes
Protein Subunits
G1 Phase
Metaphase
Ligases
Ubiquitin
Saccharomyces cerevisiae
Cell Cycle
Proteins
Peptides

ASJC Scopus subject areas

  • General

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Yu, H., Peters, J. M., King, R. W., Page, A. M., Hieter, P., & Kirschner, M. W. (1998). Identification of a cullin homology region in a subunit of the anaphase- promoting complex. Science, 279(5354), 1219-1222. https://doi.org/10.1126/science.279.5354.1219

Identification of a cullin homology region in a subunit of the anaphase- promoting complex. / Yu, Hongtao; Peters, Jan Michael; King, Randall W.; Page, Andrew M.; Hieter, Philip; Kirschner, Marc W.

In: Science, Vol. 279, No. 5354, 20.02.1998, p. 1219-1222.

Research output: Contribution to journalArticle

Yu, H, Peters, JM, King, RW, Page, AM, Hieter, P & Kirschner, MW 1998, 'Identification of a cullin homology region in a subunit of the anaphase- promoting complex', Science, vol. 279, no. 5354, pp. 1219-1222. https://doi.org/10.1126/science.279.5354.1219
Yu, Hongtao ; Peters, Jan Michael ; King, Randall W. ; Page, Andrew M. ; Hieter, Philip ; Kirschner, Marc W. / Identification of a cullin homology region in a subunit of the anaphase- promoting complex. In: Science. 1998 ; Vol. 279, No. 5354. pp. 1219-1222.
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