Identification of a DNase activated in Xenopus egg extracts undergoing apoptosis

Shan Zhu, Zhengfan Jiang, Bo Zhang, Zhigang Lu, Zhonghe Zhai

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

A cell-free apoptosis system was established by adding dATP and cytochrome c to Xenopus laevis egg extracts S-150. Accompanied by an incubation process, an apoptosis-specific DNase was activated in egg extracts which depended on Mg2+ and inhibited by Zn2+ . Two nucleases existing in egg extracts were revealed by in-gel nuclease assay. Further experiments showed that 27 ku nuclease which was different from other Ca2+/Mg2+-dependent nucleuses was a possible candidate involved in apoptosis.

Original languageEnglish (US)
Pages (from-to)522-526
Number of pages5
JournalChinese Science Bulletin
Volume43
Issue number6
DOIs
StatePublished - Jan 1 1998

Keywords

  • Apoptosis
  • Cell-free system
  • Dnase
  • Xenopus egg extracts

ASJC Scopus subject areas

  • General

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