Identification of a Drosophila G protein α subunit (dGqα-3) expressed in chemosensory cells and central neurons

Subha Talluri, Anand Bhatt, Dean P. Smith

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71 Citations (Scopus)

Abstract

We have identified another Drosophila GTP-binding protein (G protein) α subunit, dGqα-3. Transcripts encoding dGqα-3 are derived from alternative splicing of the dGqα locus previously shown to encode two visual-system-specific transcripts [Lee, Y.-J., Dobbs, M.B., Verardi, M.L. & Hyde, D.R. (1990) Neuron 5, 889-898]. Immunolocalization studies using dGqα-3 isoform-specific antibodies and LacZ fusion genes show that dGqα-3 is expressed in chemosensory cells of the olfactory and taste structures, including a subset of olfactory and gustatory neurons, and in cells of the central nervous system, including neurons in the lamina ganglionaris. These data are consistent with a variety of roles for dGqα-3, including mediating a subset of olfactory and gustatory responses in Drosophila, and supports the idea that some chemosensory responses use G protein-coupled receptors and the second messenger inositol 1,4,5-trisphosphate.

Original languageEnglish (US)
Pages (from-to)11475-11479
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume92
Issue number25
StatePublished - Dec 5 1995

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Protein Subunits
GTP-Binding Proteins
Neurons
Drosophila Proteins
Lac Operon
Inositol 1,4,5-Trisphosphate
Alternative Splicing
Second Messenger Systems
G-Protein-Coupled Receptors
Drosophila
Protein Isoforms
Central Nervous System
Antibodies
Drosophila garnet protein

Keywords

  • GTP-binding protein
  • Olfaction
  • RNA
  • Signal transduction

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

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abstract = "We have identified another Drosophila GTP-binding protein (G protein) α subunit, dGqα-3. Transcripts encoding dGqα-3 are derived from alternative splicing of the dGqα locus previously shown to encode two visual-system-specific transcripts [Lee, Y.-J., Dobbs, M.B., Verardi, M.L. & Hyde, D.R. (1990) Neuron 5, 889-898]. Immunolocalization studies using dGqα-3 isoform-specific antibodies and LacZ fusion genes show that dGqα-3 is expressed in chemosensory cells of the olfactory and taste structures, including a subset of olfactory and gustatory neurons, and in cells of the central nervous system, including neurons in the lamina ganglionaris. These data are consistent with a variety of roles for dGqα-3, including mediating a subset of olfactory and gustatory responses in Drosophila, and supports the idea that some chemosensory responses use G protein-coupled receptors and the second messenger inositol 1,4,5-trisphosphate.",
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AU - Bhatt, Anand

AU - Smith, Dean P.

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N2 - We have identified another Drosophila GTP-binding protein (G protein) α subunit, dGqα-3. Transcripts encoding dGqα-3 are derived from alternative splicing of the dGqα locus previously shown to encode two visual-system-specific transcripts [Lee, Y.-J., Dobbs, M.B., Verardi, M.L. & Hyde, D.R. (1990) Neuron 5, 889-898]. Immunolocalization studies using dGqα-3 isoform-specific antibodies and LacZ fusion genes show that dGqα-3 is expressed in chemosensory cells of the olfactory and taste structures, including a subset of olfactory and gustatory neurons, and in cells of the central nervous system, including neurons in the lamina ganglionaris. These data are consistent with a variety of roles for dGqα-3, including mediating a subset of olfactory and gustatory responses in Drosophila, and supports the idea that some chemosensory responses use G protein-coupled receptors and the second messenger inositol 1,4,5-trisphosphate.

AB - We have identified another Drosophila GTP-binding protein (G protein) α subunit, dGqα-3. Transcripts encoding dGqα-3 are derived from alternative splicing of the dGqα locus previously shown to encode two visual-system-specific transcripts [Lee, Y.-J., Dobbs, M.B., Verardi, M.L. & Hyde, D.R. (1990) Neuron 5, 889-898]. Immunolocalization studies using dGqα-3 isoform-specific antibodies and LacZ fusion genes show that dGqα-3 is expressed in chemosensory cells of the olfactory and taste structures, including a subset of olfactory and gustatory neurons, and in cells of the central nervous system, including neurons in the lamina ganglionaris. These data are consistent with a variety of roles for dGqα-3, including mediating a subset of olfactory and gustatory responses in Drosophila, and supports the idea that some chemosensory responses use G protein-coupled receptors and the second messenger inositol 1,4,5-trisphosphate.

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