Identification of a human cDNA encoding a protein that is structurally and functionally related to the yeast adenylyl cyclase-associated CAP proteins

Heather Matviw, Gang Yu, Dallan Young

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

The adenylyl cyclases of both Saccharomyces cerevisiae and Schizosaccharomyces pombe are associated with related proteins named CAP. In S. cerevisiae, CAP is required for cellular responses mediated by the RAS/cyclic AMP pathway. Both yeast CAPs appear to be bifunctional proteins: the N-terminal domains are required for the proper function of adenylyl cyclase, while loss of the C-terminal domains results in morphological and nutritional defects that appear to be unrelated to the cAMP pathways. Expression of either yeast CAP in the heterologous yeast suppresses phenotypes associated with loss of the C-terminal domain of the endogenous CAP but does not suppress loss of the N-terminal domain. On the basis of the homology between the two yeast CAP proteins, we have designed degenerate oligonucleotides that we used to detect, by the polymerase chain reaction method, a human cDNA fragment encoding a CAP-related peptide. Using the polymerase chain reaction fragment as a probe, we isolated a human cDNA clone encoding a 475-amino-acid protein that is homologous to the yeast CAP proteins. Expression of the human CAP protein in S. cerevisiae suppresses the phenotypes associated with loss of the C-terminal domain of CAP but does not suppress phenotypes associated with loss of the N-terminal domain. Thus, CAP proteins have been structurally and, to some extent, functionally conserved in evolution between yeasts and mammals.

Original languageEnglish (US)
Pages (from-to)5033-5040
Number of pages8
JournalMolecular and Cellular Biology
Volume12
Issue number11
StatePublished - Nov 1992

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Forensic Anthropology
Adenylyl Cyclases
Complementary DNA
Yeasts
Fungal Proteins
Phenotype
Saccharomyces cerevisiae
Proteins
Saccharomyces cerevisiae Proteins
Polymerase Chain Reaction
Schizosaccharomyces
Oligonucleotides
Cyclic AMP
Mammals
Clone Cells
Amino Acids
Peptides

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Identification of a human cDNA encoding a protein that is structurally and functionally related to the yeast adenylyl cyclase-associated CAP proteins. / Matviw, Heather; Yu, Gang; Young, Dallan.

In: Molecular and Cellular Biology, Vol. 12, No. 11, 11.1992, p. 5033-5040.

Research output: Contribution to journalArticle

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