Identification of a human cDNA sequence which encodes a novel membrane-associated protein containing a zinc metalloprotease motif

Ying Chun Bao, Hiromichi Tsuruga, Momoki Hirai, Kazuki Yasuda, Norihide Yokoi, Toshio Kitamura, Hidetoshi Kumagai

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We report the cloning and characterization of a human cDNA predicted to encode a novel hydrophobic protein containing four transmembrane domains and a zinc metalloprotease motif, HEXXH, between the third and fourth transmembrane domains, and have named the molecule metalloprotease-related protein-1 (MPRP-1). The MPRP-1 gene was localized to chromosome 1-p32.3 by radiation hybrid mapping, and Northern blot analysis revealed expression in many organs, with strong expression in the heart, skeletal muscle, kidney and liver. Immunohistochemical analyisis showed that MPRP-1 was localized in the endoplasmic reticulum (ER), and not in the Golgi compartment. Fragments of DNA encoding a segment homologous to the HEXXH motif of MPRP-1 are widely found in bacteria, yeast, plants, and animals. These results suggest that the MPRP-1 may have highly conserved functions, such as in intracellular proteolytic processing in the ER.

Original languageEnglish (US)
Pages (from-to)123-128
Number of pages6
JournalDNA Research
Volume10
Issue number3
DOIs
StatePublished - 2003

Fingerprint

Forensic Anthropology
Metalloproteases
Zinc
Membrane Proteins
Complementary DNA
Proteins
Endoplasmic Reticulum
Radiation Hybrid Mapping
Chromosomes, Human, Pair 1
Northern Blotting
Organism Cloning
Myocardium
Skeletal Muscle
Yeasts
Bacteria
Kidney
Liver
DNA

Keywords

  • Endoplasmic reticulum
  • Membrane protein
  • Metalloprotease

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

Identification of a human cDNA sequence which encodes a novel membrane-associated protein containing a zinc metalloprotease motif. / Bao, Ying Chun; Tsuruga, Hiromichi; Hirai, Momoki; Yasuda, Kazuki; Yokoi, Norihide; Kitamura, Toshio; Kumagai, Hidetoshi.

In: DNA Research, Vol. 10, No. 3, 2003, p. 123-128.

Research output: Contribution to journalArticle

Bao, Ying Chun ; Tsuruga, Hiromichi ; Hirai, Momoki ; Yasuda, Kazuki ; Yokoi, Norihide ; Kitamura, Toshio ; Kumagai, Hidetoshi. / Identification of a human cDNA sequence which encodes a novel membrane-associated protein containing a zinc metalloprotease motif. In: DNA Research. 2003 ; Vol. 10, No. 3. pp. 123-128.
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AU - Yokoi, Norihide

AU - Kitamura, Toshio

AU - Kumagai, Hidetoshi

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