Identification of a new polypeptide coded by reovirus gene S1

G. Sarkar, J. Pelletier, R. Bassel-Duby, A. Jayasuriya, B. N. Fields, N. Sonenberg

Research output: Contribution to journalArticle

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Abstract

The reovirus S1 gene has recently been shown potentially to encode two polypeptides (from two overlapping reading frames) having predicted molecular weights of 49,071 and 16,143. The larger polypeptide is reovirus protein σ 1, but synthesis of the smaller polypeptide has not been described to date. A truncated clone of the S1 gene in which the first ATG is deleted was expressed in an in vitro protein synthesis system to yield a ~13-kilodalton polypeptide, as determined from migration on sodium dodecyl sulfate-polyacrylamide gels. A polypeptide with a similar migration pattern on sodium dodecyl sulfate-polyacrylamide gels was present in reovirus-infected cells and absent from mock-infected cells. Comparative tryptic peptide analysis of the 13-kilodalton polypeptides produced in vivo and in vitro showed them to be identical. Thus, the s1 mRNA of reovirus type 3 is apparently bicistronic, and suggests that the ~13-kilodalton polypeptide be called σ s (standing for σ small).

Original languageEnglish (US)
Pages (from-to)720-725
Number of pages6
JournalJournal of Virology
Volume54
Issue number3
StatePublished - 1985

Fingerprint

Reoviridae
polypeptides
Peptides
Genes
genes
sodium dodecyl sulfate
polyacrylamide
Sodium Dodecyl Sulfate
gels
Mammalian Orthoreovirus
Mammalian orthoreovirus 3
Reading Frames
Proteins
peptides
cells
clones
molecular weight
Clone Cells
Molecular Weight
synthesis

ASJC Scopus subject areas

  • Immunology

Cite this

Sarkar, G., Pelletier, J., Bassel-Duby, R., Jayasuriya, A., Fields, B. N., & Sonenberg, N. (1985). Identification of a new polypeptide coded by reovirus gene S1. Journal of Virology, 54(3), 720-725.

Identification of a new polypeptide coded by reovirus gene S1. / Sarkar, G.; Pelletier, J.; Bassel-Duby, R.; Jayasuriya, A.; Fields, B. N.; Sonenberg, N.

In: Journal of Virology, Vol. 54, No. 3, 1985, p. 720-725.

Research output: Contribution to journalArticle

Sarkar, G, Pelletier, J, Bassel-Duby, R, Jayasuriya, A, Fields, BN & Sonenberg, N 1985, 'Identification of a new polypeptide coded by reovirus gene S1', Journal of Virology, vol. 54, no. 3, pp. 720-725.
Sarkar G, Pelletier J, Bassel-Duby R, Jayasuriya A, Fields BN, Sonenberg N. Identification of a new polypeptide coded by reovirus gene S1. Journal of Virology. 1985;54(3):720-725.
Sarkar, G. ; Pelletier, J. ; Bassel-Duby, R. ; Jayasuriya, A. ; Fields, B. N. ; Sonenberg, N. / Identification of a new polypeptide coded by reovirus gene S1. In: Journal of Virology. 1985 ; Vol. 54, No. 3. pp. 720-725.
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