Identification of a novel tumor necrosis factor α/cachectin from the livers of burned and infected rats

C. Keogh, Y. Fong, M. A. Marano, S. Seniuk, W. He, A. Barber, J. P. Minei, D. Felsen, S. F. Lowry, L. L. Moldawer

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Abstract

Tumor necrosis factor α (TNF-α)/cachectin is a monocyte/macrophage-derived cytokine implicated as a proximal mediator of many of the catastrophic host responses to infection or endotoxin. However, circulating levels of TNF-α/cachectin have only been episodically detected in hospitalized patients with life-threatening bacterial infections. In the present report, increased quantities of immune-reactive TNF-α/cachectin were recovered from the livers of rats 3 days following a lethal burn and infection. Two species of TNF-α/cachectin were detected, one of approximately 29 kd and the other 17 kd, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. In murine peritoneal macrophages and rat Kupffer cells stimulated in vitro with endotoxin, a 29-kd cell-associated and 17-kd secreted form were also detected. We conclude that the increased appearance in vivo of a 29-kd form of TNF-α/cachectin from the livers of lethally burned and infected rats represents a novel cell-associated form of the protein.

Original languageEnglish (US)
Pages (from-to)79-85
Number of pages7
JournalArchives of Surgery
Volume125
Issue number1
Publication statusPublished - 1990

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ASJC Scopus subject areas

  • Surgery

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Keogh, C., Fong, Y., Marano, M. A., Seniuk, S., He, W., Barber, A., ... Moldawer, L. L. (1990). Identification of a novel tumor necrosis factor α/cachectin from the livers of burned and infected rats. Archives of Surgery, 125(1), 79-85.