Identification of a peptide inhibitor of the cardiac sarcolemmal Na+-Ca2+ exchanger

Zhaoping Li, Debora A. Nicoll, Anthony Collins, Donald W. Hilgemann, Adelaida G. Filoteo, John T. Penniston, James N. Weise, John M. Tomich, Kenneth D. Philipson

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Abstract

The deduced amino acid sequence of the cardiac sarcolemmal Na+-Ca2+ exchanger has a region which could represent a calmodulin binding site. As calmodulin binding regions of proteins often have an autoinhibitory role, a synthetic peptide with this sequence was tested for functional effects on Na+-Ca2+ exchange activity. The peptide inhibits the Na+-dependent Ca2+ uptake (K1 ∼ 1.5 μM) and the Na+ o-dependent Ca2+ efflux of sarcolemmal vesicles in a noncompetitive manner with respect to both Na+ and Ca2+. The peptide is also a potent inhibitor (K1 ∼ 0.1 μM) of the Na+-Ca2+ exchange current of excised sarcolemmal patches. The binding site for the peptide on the exchanger is on the cytoplasmic surface of the membrane. The exchanger inhibitory peptide binds calmodulin with a moderately high affinity. From the characteristics of the inhibition of the exchange of sarcolemmal vesicles, we deduce that only inside-out sarcolemmal vesicles participate in the usual Na+-Ca2+ exchange assay. This contrasts with the common assumption that both inside-out and right-side-out vesicles exhibit exchange activity.

Original languageEnglish (US)
Pages (from-to)1014-1020
Number of pages7
JournalJournal of Biological Chemistry
Volume266
Issue number2
Publication statusPublished - Jan 15 1991

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ASJC Scopus subject areas

  • Biochemistry

Cite this

Li, Z., Nicoll, D. A., Collins, A., Hilgemann, D. W., Filoteo, A. G., Penniston, J. T., ... Philipson, K. D. (1991). Identification of a peptide inhibitor of the cardiac sarcolemmal Na+-Ca2+ exchanger. Journal of Biological Chemistry, 266(2), 1014-1020.