Identification of a phosphodiesterase that converts inositol cyclic 1: 2-Phosphate to inositol 2-phosphate

Theodora S. Ross, Philip W. Majerus

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Inositol 2-phosphate (Ins(2)P) has been identified in several cell types. The cellular levels of Ins(2)P appear to be directly correlated with the levels of inositol 1:2-cyclic phosphate (cIns(1:2)P) (Ross, T. S., Wang, F. P., and Majerus, P. W. (1992) J. Biol. Chem. 267, 19919-19923). In this study we have detected an enzyme in extracts from CV-1 cells and rat cerebellum that converts cIns(1:2)P to Ins(2)P and inositol 1-phosphate. This enzyme (designated cyclic hydrolase II) is not the same protein previously designated cIns(1:2)P 2-phosphohydrolase (cyclic hydrolase I). The products, heat inactivation curves, pH optima, and metal dependence of these two activities are different, and the two activities were separated by DEAE and gel filtration chromatography. Mixing of cyclic hydrolase I with cyclic hydrolase II does not effect the activity of either. The Km, of the CV-1 cyclic hydrolase II for D-cIns(1:2)P is 10 μM. The enzyme is approximately 55 kDa as estimated by gel filtration analysis in the presence of sodium chloride and 120 kDa in its absence.

Original languageEnglish (US)
Pages (from-to)19924-19928
Number of pages5
JournalJournal of Biological Chemistry
Volume267
Issue number28
StatePublished - Oct 5 1992

Fingerprint

Phosphoric Diester Hydrolases
Hydrolases
Inositol
Phosphates
Gel Chromatography
Enzymes
Gels
Chromatography
Phosphoric Monoester Hydrolases
Sodium Chloride
Cerebellum
Rats
Hot Temperature
Metals
inositol 2-monophosphate
Proteins
1,2-diamino-1,2-N,N'-carbonyl-1,2-dideoxyglucose hydrate

ASJC Scopus subject areas

  • Biochemistry

Cite this

Identification of a phosphodiesterase that converts inositol cyclic 1 : 2-Phosphate to inositol 2-phosphate. / Ross, Theodora S.; Majerus, Philip W.

In: Journal of Biological Chemistry, Vol. 267, No. 28, 05.10.1992, p. 19924-19928.

Research output: Contribution to journalArticle

@article{030373a672dc4f63bf1bee51fa58a15b,
title = "Identification of a phosphodiesterase that converts inositol cyclic 1: 2-Phosphate to inositol 2-phosphate",
abstract = "Inositol 2-phosphate (Ins(2)P) has been identified in several cell types. The cellular levels of Ins(2)P appear to be directly correlated with the levels of inositol 1:2-cyclic phosphate (cIns(1:2)P) (Ross, T. S., Wang, F. P., and Majerus, P. W. (1992) J. Biol. Chem. 267, 19919-19923). In this study we have detected an enzyme in extracts from CV-1 cells and rat cerebellum that converts cIns(1:2)P to Ins(2)P and inositol 1-phosphate. This enzyme (designated cyclic hydrolase II) is not the same protein previously designated cIns(1:2)P 2-phosphohydrolase (cyclic hydrolase I). The products, heat inactivation curves, pH optima, and metal dependence of these two activities are different, and the two activities were separated by DEAE and gel filtration chromatography. Mixing of cyclic hydrolase I with cyclic hydrolase II does not effect the activity of either. The Km, of the CV-1 cyclic hydrolase II for D-cIns(1:2)P is 10 μM. The enzyme is approximately 55 kDa as estimated by gel filtration analysis in the presence of sodium chloride and 120 kDa in its absence.",
author = "Ross, {Theodora S.} and Majerus, {Philip W.}",
year = "1992",
month = "10",
day = "5",
language = "English (US)",
volume = "267",
pages = "19924--19928",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "28",

}

TY - JOUR

T1 - Identification of a phosphodiesterase that converts inositol cyclic 1

T2 - 2-Phosphate to inositol 2-phosphate

AU - Ross, Theodora S.

AU - Majerus, Philip W.

PY - 1992/10/5

Y1 - 1992/10/5

N2 - Inositol 2-phosphate (Ins(2)P) has been identified in several cell types. The cellular levels of Ins(2)P appear to be directly correlated with the levels of inositol 1:2-cyclic phosphate (cIns(1:2)P) (Ross, T. S., Wang, F. P., and Majerus, P. W. (1992) J. Biol. Chem. 267, 19919-19923). In this study we have detected an enzyme in extracts from CV-1 cells and rat cerebellum that converts cIns(1:2)P to Ins(2)P and inositol 1-phosphate. This enzyme (designated cyclic hydrolase II) is not the same protein previously designated cIns(1:2)P 2-phosphohydrolase (cyclic hydrolase I). The products, heat inactivation curves, pH optima, and metal dependence of these two activities are different, and the two activities were separated by DEAE and gel filtration chromatography. Mixing of cyclic hydrolase I with cyclic hydrolase II does not effect the activity of either. The Km, of the CV-1 cyclic hydrolase II for D-cIns(1:2)P is 10 μM. The enzyme is approximately 55 kDa as estimated by gel filtration analysis in the presence of sodium chloride and 120 kDa in its absence.

AB - Inositol 2-phosphate (Ins(2)P) has been identified in several cell types. The cellular levels of Ins(2)P appear to be directly correlated with the levels of inositol 1:2-cyclic phosphate (cIns(1:2)P) (Ross, T. S., Wang, F. P., and Majerus, P. W. (1992) J. Biol. Chem. 267, 19919-19923). In this study we have detected an enzyme in extracts from CV-1 cells and rat cerebellum that converts cIns(1:2)P to Ins(2)P and inositol 1-phosphate. This enzyme (designated cyclic hydrolase II) is not the same protein previously designated cIns(1:2)P 2-phosphohydrolase (cyclic hydrolase I). The products, heat inactivation curves, pH optima, and metal dependence of these two activities are different, and the two activities were separated by DEAE and gel filtration chromatography. Mixing of cyclic hydrolase I with cyclic hydrolase II does not effect the activity of either. The Km, of the CV-1 cyclic hydrolase II for D-cIns(1:2)P is 10 μM. The enzyme is approximately 55 kDa as estimated by gel filtration analysis in the presence of sodium chloride and 120 kDa in its absence.

UR - http://www.scopus.com/inward/record.url?scp=0026701680&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026701680&partnerID=8YFLogxK

M3 - Article

C2 - 1328187

AN - SCOPUS:0026701680

VL - 267

SP - 19924

EP - 19928

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 28

ER -