Identification of a second transforming gene, ras(n), in a human multiple myeloma line with a rearranged c-myc allele

T. J. Ernst, A. Gazdar, J. Ritz, M. A. Shipp

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Multiple myeloma is a disease characterized by a long, slowly progressive phase and a final, more aggressive one. Little is known about the mechanism of transformation of myeloma cells, although the clinical characteristics of the disease suggest a multi-step process. Recently, a myeloma cell line, NCl-H929, was isolated from a patient with aggressive preterminal disease and found to have a rearranged myc allele. This myeloma cell line has been further characterized in a focus formation assay to determine whether its unusual growth characteristics were associated with a second activated transforming gene. We now report that the NCl-H929 myeloma cell line has an activated ras(n) allele in addition to a rearranged myc allele. This is the first identification of an activated transforming gene in a multiple myeloma cell line; furthermore, the characterization of two independently activated oncogenes in this B cell malignancy has implications for both the pathogenesis and evolution of the disease.

Original languageEnglish (US)
Pages (from-to)1163-1167
Number of pages5
JournalBlood
Volume72
Issue number4
DOIs
StatePublished - 1988

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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