TY - JOUR
T1 - Identification of a second transforming gene, ras(n), in a human multiple myeloma line with a rearranged c-myc allele
AU - Ernst, T. J.
AU - Gazdar, A.
AU - Ritz, J.
AU - Shipp, M. A.
PY - 1988
Y1 - 1988
N2 - Multiple myeloma is a disease characterized by a long, slowly progressive phase and a final, more aggressive one. Little is known about the mechanism of transformation of myeloma cells, although the clinical characteristics of the disease suggest a multi-step process. Recently, a myeloma cell line, NCl-H929, was isolated from a patient with aggressive preterminal disease and found to have a rearranged myc allele. This myeloma cell line has been further characterized in a focus formation assay to determine whether its unusual growth characteristics were associated with a second activated transforming gene. We now report that the NCl-H929 myeloma cell line has an activated ras(n) allele in addition to a rearranged myc allele. This is the first identification of an activated transforming gene in a multiple myeloma cell line; furthermore, the characterization of two independently activated oncogenes in this B cell malignancy has implications for both the pathogenesis and evolution of the disease.
AB - Multiple myeloma is a disease characterized by a long, slowly progressive phase and a final, more aggressive one. Little is known about the mechanism of transformation of myeloma cells, although the clinical characteristics of the disease suggest a multi-step process. Recently, a myeloma cell line, NCl-H929, was isolated from a patient with aggressive preterminal disease and found to have a rearranged myc allele. This myeloma cell line has been further characterized in a focus formation assay to determine whether its unusual growth characteristics were associated with a second activated transforming gene. We now report that the NCl-H929 myeloma cell line has an activated ras(n) allele in addition to a rearranged myc allele. This is the first identification of an activated transforming gene in a multiple myeloma cell line; furthermore, the characterization of two independently activated oncogenes in this B cell malignancy has implications for both the pathogenesis and evolution of the disease.
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U2 - 10.1182/blood.v72.4.1163.bloodjournal7241163
DO - 10.1182/blood.v72.4.1163.bloodjournal7241163
M3 - Article
C2 - 3048435
AN - SCOPUS:0023790821
SN - 0006-4971
VL - 72
SP - 1163
EP - 1167
JO - Blood
JF - Blood
IS - 4
ER -