Identification of a tyrosine residue in the saccharide binding site of ricin B-chain using N-[14C]acetylimidazole

Edward J. Wawrzynczak, Anna Falasca, William A. Jeffery, Graham J. Watson, Philip E. Thorpe

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The binding of ricin B-chain to Sepharose, a galactose-based adsorbent, was reversibly inactivated by acetylation of tyrosine residues in the absence of lactose. In the presence of lactose, two tyrosine residues were protected against modification and the B-chain retained its binding ability. Analyses of tryptic peptides from B-chain modified with N-[14C]acetylimidazole in the presence and absence of lactose showed that Tyr-248 is present in one of the galactose-binding sites.

Original languageEnglish (US)
Pages (from-to)51-55
Number of pages5
JournalFEBS Letters
Volume219
Issue number1
DOIs
StatePublished - Jul 13 1987

Fingerprint

Ricin
Lactose
Tyrosine
Binding Sites
Galactose
Acetylation
Sepharose
Adsorbents
N-acetylimidazole

Keywords

  • B-chain
  • N-[C]Acetylimidazole
  • Ricin
  • Saccharide binding
  • Tyrosine residue

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Identification of a tyrosine residue in the saccharide binding site of ricin B-chain using N-[14C]acetylimidazole. / Wawrzynczak, Edward J.; Falasca, Anna; Jeffery, William A.; Watson, Graham J.; Thorpe, Philip E.

In: FEBS Letters, Vol. 219, No. 1, 13.07.1987, p. 51-55.

Research output: Contribution to journalArticle

Wawrzynczak, Edward J. ; Falasca, Anna ; Jeffery, William A. ; Watson, Graham J. ; Thorpe, Philip E. / Identification of a tyrosine residue in the saccharide binding site of ricin B-chain using N-[14C]acetylimidazole. In: FEBS Letters. 1987 ; Vol. 219, No. 1. pp. 51-55.
@article{c086e3c8b03c41b4ba6d2cfba8bc6cd2,
title = "Identification of a tyrosine residue in the saccharide binding site of ricin B-chain using N-[14C]acetylimidazole",
abstract = "The binding of ricin B-chain to Sepharose, a galactose-based adsorbent, was reversibly inactivated by acetylation of tyrosine residues in the absence of lactose. In the presence of lactose, two tyrosine residues were protected against modification and the B-chain retained its binding ability. Analyses of tryptic peptides from B-chain modified with N-[14C]acetylimidazole in the presence and absence of lactose showed that Tyr-248 is present in one of the galactose-binding sites.",
keywords = "B-chain, N-[C]Acetylimidazole, Ricin, Saccharide binding, Tyrosine residue",
author = "Wawrzynczak, {Edward J.} and Anna Falasca and Jeffery, {William A.} and Watson, {Graham J.} and Thorpe, {Philip E.}",
year = "1987",
month = "7",
day = "13",
doi = "10.1016/0014-5793(87)81189-4",
language = "English (US)",
volume = "219",
pages = "51--55",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Identification of a tyrosine residue in the saccharide binding site of ricin B-chain using N-[14C]acetylimidazole

AU - Wawrzynczak, Edward J.

AU - Falasca, Anna

AU - Jeffery, William A.

AU - Watson, Graham J.

AU - Thorpe, Philip E.

PY - 1987/7/13

Y1 - 1987/7/13

N2 - The binding of ricin B-chain to Sepharose, a galactose-based adsorbent, was reversibly inactivated by acetylation of tyrosine residues in the absence of lactose. In the presence of lactose, two tyrosine residues were protected against modification and the B-chain retained its binding ability. Analyses of tryptic peptides from B-chain modified with N-[14C]acetylimidazole in the presence and absence of lactose showed that Tyr-248 is present in one of the galactose-binding sites.

AB - The binding of ricin B-chain to Sepharose, a galactose-based adsorbent, was reversibly inactivated by acetylation of tyrosine residues in the absence of lactose. In the presence of lactose, two tyrosine residues were protected against modification and the B-chain retained its binding ability. Analyses of tryptic peptides from B-chain modified with N-[14C]acetylimidazole in the presence and absence of lactose showed that Tyr-248 is present in one of the galactose-binding sites.

KW - B-chain

KW - N-[C]Acetylimidazole

KW - Ricin

KW - Saccharide binding

KW - Tyrosine residue

UR - http://www.scopus.com/inward/record.url?scp=0023229356&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023229356&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(87)81189-4

DO - 10.1016/0014-5793(87)81189-4

M3 - Article

VL - 219

SP - 51

EP - 55

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 1

ER -