Identification of a tyrosine residue in the saccharide binding site of ricin B-chain using N-[14C]acetylimidazole

Edward J. Wawrzynczak; Anna Falasca; William A. Jeffery; Graham J. Watson; Philip E. Thorpe

doi:10.1016/0014-5793(87)81189-4

Identification of a tyrosine residue in the saccharide binding site of ricin B-chain using N-[¹⁴C]acetylimidazole

Edward J. Wawrzynczak, Anna Falasca, William A. Jeffery, Graham J. Watson, Philip E. Thorpe

Pharmacology

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The binding of ricin B-chain to Sepharose, a galactose-based adsorbent, was reversibly inactivated by acetylation of tyrosine residues in the absence of lactose. In the presence of lactose, two tyrosine residues were protected against modification and the B-chain retained its binding ability. Analyses of tryptic peptides from B-chain modified with N-[¹⁴C]acetylimidazole in the presence and absence of lactose showed that Tyr-248 is present in one of the galactose-binding sites.

Original language	English (US)
Pages (from-to)	51-55
Number of pages	5
Journal	FEBS Letters
Volume	219
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(87)81189-4
State	Published - Jul 13 1987

Keywords

B-chain
N-[C]Acetylimidazole
Ricin
Saccharide binding
Tyrosine residue

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1016/0014-5793(87)81189-4

Cite this

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title = "Identification of a tyrosine residue in the saccharide binding site of ricin B-chain using N-[14C]acetylimidazole",

abstract = "The binding of ricin B-chain to Sepharose, a galactose-based adsorbent, was reversibly inactivated by acetylation of tyrosine residues in the absence of lactose. In the presence of lactose, two tyrosine residues were protected against modification and the B-chain retained its binding ability. Analyses of tryptic peptides from B-chain modified with N-[14C]acetylimidazole in the presence and absence of lactose showed that Tyr-248 is present in one of the galactose-binding sites.",

keywords = "B-chain, N-[C]Acetylimidazole, Ricin, Saccharide binding, Tyrosine residue",

author = "Wawrzynczak, {Edward J.} and Anna Falasca and Jeffery, {William A.} and Watson, {Graham J.} and Thorpe, {Philip E.}",

note = "Funding Information: We thank Mr Rob Philip for amino acid analysis and Mr Nick Totty for amino acid sequencing. We also thank Dr M. Crumpton for valuable com- ments on the manuscript. A.F. wishes to thank the European Molecular Biology Organisation for the award of a Fellowship.",

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T1 - Identification of a tyrosine residue in the saccharide binding site of ricin B-chain using N-[14C]acetylimidazole

AU - Wawrzynczak, Edward J.

AU - Falasca, Anna

AU - Jeffery, William A.

AU - Watson, Graham J.

AU - Thorpe, Philip E.

N1 - Funding Information: We thank Mr Rob Philip for amino acid analysis and Mr Nick Totty for amino acid sequencing. We also thank Dr M. Crumpton for valuable com- ments on the manuscript. A.F. wishes to thank the European Molecular Biology Organisation for the award of a Fellowship.

PY - 1987/7/13

Y1 - 1987/7/13

N2 - The binding of ricin B-chain to Sepharose, a galactose-based adsorbent, was reversibly inactivated by acetylation of tyrosine residues in the absence of lactose. In the presence of lactose, two tyrosine residues were protected against modification and the B-chain retained its binding ability. Analyses of tryptic peptides from B-chain modified with N-[14C]acetylimidazole in the presence and absence of lactose showed that Tyr-248 is present in one of the galactose-binding sites.

AB - The binding of ricin B-chain to Sepharose, a galactose-based adsorbent, was reversibly inactivated by acetylation of tyrosine residues in the absence of lactose. In the presence of lactose, two tyrosine residues were protected against modification and the B-chain retained its binding ability. Analyses of tryptic peptides from B-chain modified with N-[14C]acetylimidazole in the presence and absence of lactose showed that Tyr-248 is present in one of the galactose-binding sites.

KW - B-chain

KW - N-[C]Acetylimidazole

KW - Ricin

KW - Saccharide binding

KW - Tyrosine residue

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