Identification of ATP synthase as a lipid peroxide protein adduct in pancreatic islets from humans with and without type 2 diabetes mellitus

M. J. MacDonald, E. C. Langberg, A. Tibell, G. Sabat, M. A. Kendrick, L. I. Szweda, C. G. Ostenson

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Context: Most current knowledge of pancreatic islet pathophysiology in diabetes mellitus has come from animal models. Even though islets from humans are readily available, only a few come from diabetic donors. We had the uncommon opportunity to acquire islets from humans with type 2 diabetes and used it to perform a study not previously done with human or animal islets. Objectives: Oxidative stress has been proposed as a mechanism for impaired β-cell function in type 2 diabetes. Lipid peroxides caused by reactive oxygen species are damaging to body tissues. The objective was to determine whether lipid peroxide-protein adducts occur in pancreatic islets of humans with type 2 diabetes. Design: Immunoblots with two antibodies to hydroxynonenal and 2 other antibodies we generated against reactivesmall aliphaticcompounds were used todetect lipid peroxide-protein adducts in islets of patients with type 2 diabetes and controls. Results: The antibodies reacted strongly to ≥5 islet proteins. The major hydroxynonenal adduct in the islets of type 2 diabetes patients was a 52-kDa protein seen with all 4 antibodies that was also seen in islets of nondiabetic humans, rat islets, and insulinoma cells and in mitochondria of various rat tissues. Nano-LC-MS/MS (liquid chromatography-tandem mass spectrometry) and MALDI-TOF (matrix-assisted laser desorption/ionization- time of flight) analysis identified the protein as the β-chain of the mitochondrial F-ATP synthase, an enzyme responsible for 95% of ATP formed in tissues. Conclusions: Lipid peroxide-protein adducts occur in β-cells in the nondiabetic state and in diabetes. Lipid peroxidation is thought to be damaging to tissues. Analogous to various other unhealthy characteristics, the presence in nondiabetic individuals of lipid peroxide-protein adducts does not necessarily indicate they are not detrimental.

Original languageEnglish (US)
JournalJournal of Clinical Endocrinology and Metabolism
Volume98
Issue number4
DOIs
StatePublished - Apr 1 2013

Fingerprint

Lipid Peroxides
Medical problems
Islets of Langerhans
Type 2 Diabetes Mellitus
Adenosine Triphosphate
Proteins
Tissue
Antibodies
Rats
Animals
Mitochondrial Proton-Translocating ATPases
Insulinoma
Mitochondria
Oxidative stress
Liquid chromatography
Tandem Mass Spectrometry
Liquid Chromatography
Lipid Peroxidation
Ionization
Mass spectrometry

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Identification of ATP synthase as a lipid peroxide protein adduct in pancreatic islets from humans with and without type 2 diabetes mellitus. / MacDonald, M. J.; Langberg, E. C.; Tibell, A.; Sabat, G.; Kendrick, M. A.; Szweda, L. I.; Ostenson, C. G.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 98, No. 4, 01.04.2013.

Research output: Contribution to journalArticle

@article{feab7fb08e6f4a5ab4c8155374e2dad7,
title = "Identification of ATP synthase as a lipid peroxide protein adduct in pancreatic islets from humans with and without type 2 diabetes mellitus",
abstract = "Context: Most current knowledge of pancreatic islet pathophysiology in diabetes mellitus has come from animal models. Even though islets from humans are readily available, only a few come from diabetic donors. We had the uncommon opportunity to acquire islets from humans with type 2 diabetes and used it to perform a study not previously done with human or animal islets. Objectives: Oxidative stress has been proposed as a mechanism for impaired β-cell function in type 2 diabetes. Lipid peroxides caused by reactive oxygen species are damaging to body tissues. The objective was to determine whether lipid peroxide-protein adducts occur in pancreatic islets of humans with type 2 diabetes. Design: Immunoblots with two antibodies to hydroxynonenal and 2 other antibodies we generated against reactivesmall aliphaticcompounds were used todetect lipid peroxide-protein adducts in islets of patients with type 2 diabetes and controls. Results: The antibodies reacted strongly to ≥5 islet proteins. The major hydroxynonenal adduct in the islets of type 2 diabetes patients was a 52-kDa protein seen with all 4 antibodies that was also seen in islets of nondiabetic humans, rat islets, and insulinoma cells and in mitochondria of various rat tissues. Nano-LC-MS/MS (liquid chromatography-tandem mass spectrometry) and MALDI-TOF (matrix-assisted laser desorption/ionization- time of flight) analysis identified the protein as the β-chain of the mitochondrial F-ATP synthase, an enzyme responsible for 95{\%} of ATP formed in tissues. Conclusions: Lipid peroxide-protein adducts occur in β-cells in the nondiabetic state and in diabetes. Lipid peroxidation is thought to be damaging to tissues. Analogous to various other unhealthy characteristics, the presence in nondiabetic individuals of lipid peroxide-protein adducts does not necessarily indicate they are not detrimental.",
author = "MacDonald, {M. J.} and Langberg, {E. C.} and A. Tibell and G. Sabat and Kendrick, {M. A.} and Szweda, {L. I.} and Ostenson, {C. G.}",
year = "2013",
month = "4",
day = "1",
doi = "10.1210/jc.2012-4203",
language = "English (US)",
volume = "98",
journal = "Journal of Clinical Endocrinology and Metabolism",
issn = "0021-972X",
publisher = "The Endocrine Society",
number = "4",

}

TY - JOUR

T1 - Identification of ATP synthase as a lipid peroxide protein adduct in pancreatic islets from humans with and without type 2 diabetes mellitus

AU - MacDonald, M. J.

AU - Langberg, E. C.

AU - Tibell, A.

AU - Sabat, G.

AU - Kendrick, M. A.

AU - Szweda, L. I.

AU - Ostenson, C. G.

PY - 2013/4/1

Y1 - 2013/4/1

N2 - Context: Most current knowledge of pancreatic islet pathophysiology in diabetes mellitus has come from animal models. Even though islets from humans are readily available, only a few come from diabetic donors. We had the uncommon opportunity to acquire islets from humans with type 2 diabetes and used it to perform a study not previously done with human or animal islets. Objectives: Oxidative stress has been proposed as a mechanism for impaired β-cell function in type 2 diabetes. Lipid peroxides caused by reactive oxygen species are damaging to body tissues. The objective was to determine whether lipid peroxide-protein adducts occur in pancreatic islets of humans with type 2 diabetes. Design: Immunoblots with two antibodies to hydroxynonenal and 2 other antibodies we generated against reactivesmall aliphaticcompounds were used todetect lipid peroxide-protein adducts in islets of patients with type 2 diabetes and controls. Results: The antibodies reacted strongly to ≥5 islet proteins. The major hydroxynonenal adduct in the islets of type 2 diabetes patients was a 52-kDa protein seen with all 4 antibodies that was also seen in islets of nondiabetic humans, rat islets, and insulinoma cells and in mitochondria of various rat tissues. Nano-LC-MS/MS (liquid chromatography-tandem mass spectrometry) and MALDI-TOF (matrix-assisted laser desorption/ionization- time of flight) analysis identified the protein as the β-chain of the mitochondrial F-ATP synthase, an enzyme responsible for 95% of ATP formed in tissues. Conclusions: Lipid peroxide-protein adducts occur in β-cells in the nondiabetic state and in diabetes. Lipid peroxidation is thought to be damaging to tissues. Analogous to various other unhealthy characteristics, the presence in nondiabetic individuals of lipid peroxide-protein adducts does not necessarily indicate they are not detrimental.

AB - Context: Most current knowledge of pancreatic islet pathophysiology in diabetes mellitus has come from animal models. Even though islets from humans are readily available, only a few come from diabetic donors. We had the uncommon opportunity to acquire islets from humans with type 2 diabetes and used it to perform a study not previously done with human or animal islets. Objectives: Oxidative stress has been proposed as a mechanism for impaired β-cell function in type 2 diabetes. Lipid peroxides caused by reactive oxygen species are damaging to body tissues. The objective was to determine whether lipid peroxide-protein adducts occur in pancreatic islets of humans with type 2 diabetes. Design: Immunoblots with two antibodies to hydroxynonenal and 2 other antibodies we generated against reactivesmall aliphaticcompounds were used todetect lipid peroxide-protein adducts in islets of patients with type 2 diabetes and controls. Results: The antibodies reacted strongly to ≥5 islet proteins. The major hydroxynonenal adduct in the islets of type 2 diabetes patients was a 52-kDa protein seen with all 4 antibodies that was also seen in islets of nondiabetic humans, rat islets, and insulinoma cells and in mitochondria of various rat tissues. Nano-LC-MS/MS (liquid chromatography-tandem mass spectrometry) and MALDI-TOF (matrix-assisted laser desorption/ionization- time of flight) analysis identified the protein as the β-chain of the mitochondrial F-ATP synthase, an enzyme responsible for 95% of ATP formed in tissues. Conclusions: Lipid peroxide-protein adducts occur in β-cells in the nondiabetic state and in diabetes. Lipid peroxidation is thought to be damaging to tissues. Analogous to various other unhealthy characteristics, the presence in nondiabetic individuals of lipid peroxide-protein adducts does not necessarily indicate they are not detrimental.

UR - http://www.scopus.com/inward/record.url?scp=84876252001&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84876252001&partnerID=8YFLogxK

U2 - 10.1210/jc.2012-4203

DO - 10.1210/jc.2012-4203

M3 - Article

C2 - 23463654

AN - SCOPUS:84876252001

VL - 98

JO - Journal of Clinical Endocrinology and Metabolism

JF - Journal of Clinical Endocrinology and Metabolism

SN - 0021-972X

IS - 4

ER -