Identification of autoanti-Thy-1 antibody in the sera of BALB/c mice immune to P1798 lymphoma

F. S. Baechtel, M. D. Prager, E. S. Vitetta

Research output: Contribution to journalArticle

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Abstract

The specificity was established for the anti-thymocyte autoantibody which appeared in BALB/c mice (BAA) during immunization to the P1798 lymphoma. The results indicate that BAA is specific for the Thy-1 alloantigen of murine T cells. By immunoprecipitation of 125I-labeled A/J thymocyte surface antigens from freeze-thaw lysates and electrophoretic resolution of the immunoprecipitates on sodium dodecyl sulfate polyacrylamide gels, the BAA and congenic anti-Thy-1.1 or 1.2 were shown to be specific for determinants on the same antigens. Specificity of BAA for Thy-1.2 was shown also by quantitative absorption studies. Coating P1798 lymphoma cells with either BAA or anti-Thy-1.2 reduced the absorptive capacity of these cells for the same or the reciprocal antiserum. Coating P1798 with an antiserum to P1798 which was indifferent to the Thy-1.2 alloantigen did not diminish the absorptive capacity of these cells for either BAA or anti-Thy-1.2. These studies have provided firm evidence that BAA is specific for determinants on the Thy-1 alloantigen.

Original languageEnglish (US)
Pages (from-to)450-454
Number of pages5
JournalEuropean Journal of Immunology
Volume9
Issue number6
StatePublished - 1979

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Isoantigens
Lymphoma
Thymocytes
Antibodies
Immune Sera
Serum
Surface Antigens
Immunoprecipitation
Sodium Dodecyl Sulfate
Autoantibodies
Immunization
T-Lymphocytes
Antigens

ASJC Scopus subject areas

  • Immunology

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Identification of autoanti-Thy-1 antibody in the sera of BALB/c mice immune to P1798 lymphoma. / Baechtel, F. S.; Prager, M. D.; Vitetta, E. S.

In: European Journal of Immunology, Vol. 9, No. 6, 1979, p. 450-454.

Research output: Contribution to journalArticle

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AB - The specificity was established for the anti-thymocyte autoantibody which appeared in BALB/c mice (BAA) during immunization to the P1798 lymphoma. The results indicate that BAA is specific for the Thy-1 alloantigen of murine T cells. By immunoprecipitation of 125I-labeled A/J thymocyte surface antigens from freeze-thaw lysates and electrophoretic resolution of the immunoprecipitates on sodium dodecyl sulfate polyacrylamide gels, the BAA and congenic anti-Thy-1.1 or 1.2 were shown to be specific for determinants on the same antigens. Specificity of BAA for Thy-1.2 was shown also by quantitative absorption studies. Coating P1798 lymphoma cells with either BAA or anti-Thy-1.2 reduced the absorptive capacity of these cells for the same or the reciprocal antiserum. Coating P1798 with an antiserum to P1798 which was indifferent to the Thy-1.2 alloantigen did not diminish the absorptive capacity of these cells for either BAA or anti-Thy-1.2. These studies have provided firm evidence that BAA is specific for determinants on the Thy-1 alloantigen.

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