Identification of Campylobacter jejuni genes involved in commensal colonization of the chick gastrointestinal tract

David R. Hendrixson, Victor J. DiRita

Research output: Contribution to journalArticle

270 Citations (Scopus)

Abstract

Campylobacter jejuni is the leading cause of bacterial gastroenteritis in humans in developed countries throughout the world. This bacterium frequently promotes a commensal lifestyle in the gastrointestinal tracts of many animals including birds and consumption or handling of poultry meats is a prevalent source of C. jejuni for infection in humans. To understand how the bacterium promotes commensalism, we used signature-tagged transposon mutagenesis and identified 29 mutants representing 22 different genes of C. jejuni strain 81-176 involved in colonization of the chick gastrointestinal tract. Among the determinants identified were two adjacent genes, one encoding a methyl-accepting chemotaxis protein (MCP), presumably required for proper chemotaxis to a specific environmental component, and another gene encoding a putative cytochrome c peroxidase that may function to reduce periplasmic hydrogen peroxide stress during in vivo growth. Deletion of either gene resulted in attenuation for growth throughout the gastrointestinal tract. Further examination of 10 other putative MCPs or MCP-domain containing proteins of C. jejuni revealed one other required for wild-type levels of caecal colonization. This study represents one of the first genetic screens focusing on the bacterial requirements necessary for promoting commensalism in a vertebrate host.

Original languageEnglish (US)
Pages (from-to)471-484
Number of pages14
JournalMolecular Microbiology
Volume52
Issue number2
DOIs
StatePublished - Apr 2004

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Campylobacter jejuni
Gastrointestinal Tract
Symbiosis
Cytochrome-c Peroxidase
Campylobacter Infections
Genes
Gene Components
Bacteria
Gene Deletion
Gastroenteritis
Chemotaxis
Poultry
Growth
Protein C
Developed Countries
Mutagenesis
Meat
Hydrogen Peroxide
Birds
Vertebrates

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

Identification of Campylobacter jejuni genes involved in commensal colonization of the chick gastrointestinal tract. / Hendrixson, David R.; DiRita, Victor J.

In: Molecular Microbiology, Vol. 52, No. 2, 04.2004, p. 471-484.

Research output: Contribution to journalArticle

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