Identification of chlorella T-1 ingredient which enhances DMPO-OH adduct formation in Fenton reaction

M. Aktar Ali, Kikuo Oykawa, Minoru Ohwada, Tetsuya Konishi

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Chlorella T-1 is a micro algae which carries out highly active photosynthetic carbon fixation. For searching the ·OH scavenging molecules in this strain, the methanol extract of T-1 was fractionated by RP-HPLC and the fractions were analyzed for DMPO-OH formation by ESR after Fenton reaction. In the course of study, a single chlorella T-1 ingredient was found to enhance the formation of DMPO-OH rather than scavenge in the Fenton system. The ·OH enhancing chlorella component was further purified by chromatography and finally identified as lactic acid by NMR and LC-MS. From the analysis of the reaction conditions for DMPO-OH, it was found that lactic acid did not serve as direct source of ·OH but accelerated Fenton reaction.

Original languageEnglish (US)
Pages (from-to)787-797
Number of pages11
JournalBiochemistry and Molecular Biology International
Volume43
Issue number4
StatePublished - Nov 1 1997

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Keywords

  • Chlorella
  • ESR
  • Fenton reaction
  • Hydroxyl radical
  • Ingredient isolation
  • Spin trapping

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

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