Abstract
Chlorella T-1 is a micro algae which carries out highly active photosynthetic carbon fixation. For searching the ·OH scavenging molecules in this strain, the methanol extract of T-1 was fractionated by RP-HPLC and the fractions were analyzed for DMPO-OH formation by ESR after Fenton reaction. In the course of study, a single chlorella T-1 ingredient was found to enhance the formation of DMPO-OH rather than scavenge in the Fenton system. The ·OH enhancing chlorella component was further purified by chromatography and finally identified as lactic acid by NMR and LC-MS. From the analysis of the reaction conditions for DMPO-OH, it was found that lactic acid did not serve as direct source of ·OH but accelerated Fenton reaction.
Original language | English (US) |
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Pages (from-to) | 787-797 |
Number of pages | 11 |
Journal | Biochemistry and Molecular Biology International |
Volume | 43 |
Issue number | 4 |
DOIs | |
State | Published - Nov 1997 |
Keywords
- Chlorella
- ESR
- Fenton reaction
- Hydroxyl radical
- Ingredient isolation
- Spin trapping
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Genetics