Identification of chlorella T-1 ingredient which enhances DMPO-OH adduct formation in Fenton reaction

M. Aktar Ali, Kikuo Oykawa, Minoru Ohwada, Tetsuya Konishi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Chlorella T-1 is a micro algae which carries out highly active photosynthetic carbon fixation. For searching the ·OH scavenging molecules in this strain, the methanol extract of T-1 was fractionated by RP-HPLC and the fractions were analyzed for DMPO-OH formation by ESR after Fenton reaction. In the course of study, a single chlorella T-1 ingredient was found to enhance the formation of DMPO-OH rather than scavenge in the Fenton system. The ·OH enhancing chlorella component was further purified by chromatography and finally identified as lactic acid by NMR and LC-MS. From the analysis of the reaction conditions for DMPO-OH, it was found that lactic acid did not serve as direct source of ·OH but accelerated Fenton reaction.

Original languageEnglish (US)
Pages (from-to)787-797
Number of pages11
JournalBiochemistry and Molecular Biology International
Volume43
Issue number4
StatePublished - Nov 1997

Fingerprint

Chlorella
Lactic Acid
Scavenging
Photosynthesis
Algae
Chromatography
Paramagnetic resonance
Methanol
Carbon
High Pressure Liquid Chromatography
Nuclear magnetic resonance
Molecules
2,2-dimethyl-5-hydroxy-1-pyrrolidinyloxy

Keywords

  • Chlorella
  • ESR
  • Fenton reaction
  • Hydroxyl radical
  • Ingredient isolation
  • Spin trapping

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Molecular Biology

Cite this

Identification of chlorella T-1 ingredient which enhances DMPO-OH adduct formation in Fenton reaction. / Ali, M. Aktar; Oykawa, Kikuo; Ohwada, Minoru; Konishi, Tetsuya.

In: Biochemistry and Molecular Biology International, Vol. 43, No. 4, 11.1997, p. 787-797.

Research output: Contribution to journalArticle

Ali, M. Aktar ; Oykawa, Kikuo ; Ohwada, Minoru ; Konishi, Tetsuya. / Identification of chlorella T-1 ingredient which enhances DMPO-OH adduct formation in Fenton reaction. In: Biochemistry and Molecular Biology International. 1997 ; Vol. 43, No. 4. pp. 787-797.
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