Identification of direct serum-response factor gene targets during Me 2SO-induced P19 cardiac cell differentiation

Shu Xing Zhang, Eduardo Garcia-Gras, Diane R. Wycuff, Suzanne J. Marriot, Nijiati Kadeer, Wei Yu, Eric N. Olson, Daniel J. Garry, Michael S. Parmacek, Robert J. Schwartz

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Abstract

Serum-response factor (SRF) is an obligatory transcription factor, required for the formation of vertebrate mesoderm leading to the origin of the cardiovascular system. Protein A-TEV-tagged chromatin immunoprecipitation technology was used to collect direct SRF-bound gene targets from pluripotent P19 cells, induced by Me2SO treatment into an enriched cardiac cell population. From 242 sequenced DNA fragments, we identified 188 genomic DNA fragments as potential direct SRF targets that contain CArG boxes and CArG-like boxes. Of the 92 contiguous genes that were identified, a subgroup of 43 SRF targets was then further validated by co-transfection assays with SRF. Expression patterns of representative candidate genes were compared with the LacZ reporter expression activity of the endogenous SRF gene. According to the Unigene data base, 84% of the SRF target candidates were expressed, at least, in the heart. In SRF null embryonic stem cells, 81% of these SRF target candidates were greatly affected by the absence of SRF. Among these SRF-regulated genes, Raf1, Map4k4, and Bicc1 have essential roles in mesoderm formation. The 12 regulated SRF target genes, Mapk10 (JNK3), Txnl2, Azi2, Tera, Sema3a, Lrp4, Actc1, Myl3, Hspg2, Pgm2, Hif3a, and Asb5, have been implicated in cardiovascular formation, and the Ski and Hes6 genes have roles in muscle differentiation. SRF target genes related to cell mitosis and cycle, E2f5, Npm1, Cenpb, Rbbp6, and Scyl1, expressed in the heart tissue were differentially regulated in SRF null ES cells.

Original languageEnglish (US)
Pages (from-to)19115-19126
Number of pages12
JournalJournal of Biological Chemistry
Volume280
Issue number19
DOIs
StatePublished - May 13 2005

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Serum Response Factor
Cell Differentiation
Genes
Mesoderm
Semaphorin-3A
Cardiovascular system
Null Lymphocytes
Chromatin Immunoprecipitation
DNA
Staphylococcal Protein A
Embryonic Stem Cells
Cardiovascular System
Stem cells

ASJC Scopus subject areas

  • Biochemistry

Cite this

Zhang, S. X., Garcia-Gras, E., Wycuff, D. R., Marriot, S. J., Kadeer, N., Yu, W., ... Schwartz, R. J. (2005). Identification of direct serum-response factor gene targets during Me 2SO-induced P19 cardiac cell differentiation. Journal of Biological Chemistry, 280(19), 19115-19126. https://doi.org/10.1074/jbc.M413793200

Identification of direct serum-response factor gene targets during Me 2SO-induced P19 cardiac cell differentiation. / Zhang, Shu Xing; Garcia-Gras, Eduardo; Wycuff, Diane R.; Marriot, Suzanne J.; Kadeer, Nijiati; Yu, Wei; Olson, Eric N.; Garry, Daniel J.; Parmacek, Michael S.; Schwartz, Robert J.

In: Journal of Biological Chemistry, Vol. 280, No. 19, 13.05.2005, p. 19115-19126.

Research output: Contribution to journalArticle

Zhang, SX, Garcia-Gras, E, Wycuff, DR, Marriot, SJ, Kadeer, N, Yu, W, Olson, EN, Garry, DJ, Parmacek, MS & Schwartz, RJ 2005, 'Identification of direct serum-response factor gene targets during Me 2SO-induced P19 cardiac cell differentiation', Journal of Biological Chemistry, vol. 280, no. 19, pp. 19115-19126. https://doi.org/10.1074/jbc.M413793200
Zhang, Shu Xing ; Garcia-Gras, Eduardo ; Wycuff, Diane R. ; Marriot, Suzanne J. ; Kadeer, Nijiati ; Yu, Wei ; Olson, Eric N. ; Garry, Daniel J. ; Parmacek, Michael S. ; Schwartz, Robert J. / Identification of direct serum-response factor gene targets during Me 2SO-induced P19 cardiac cell differentiation. In: Journal of Biological Chemistry. 2005 ; Vol. 280, No. 19. pp. 19115-19126.
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abstract = "Serum-response factor (SRF) is an obligatory transcription factor, required for the formation of vertebrate mesoderm leading to the origin of the cardiovascular system. Protein A-TEV-tagged chromatin immunoprecipitation technology was used to collect direct SRF-bound gene targets from pluripotent P19 cells, induced by Me2SO treatment into an enriched cardiac cell population. From 242 sequenced DNA fragments, we identified 188 genomic DNA fragments as potential direct SRF targets that contain CArG boxes and CArG-like boxes. Of the 92 contiguous genes that were identified, a subgroup of 43 SRF targets was then further validated by co-transfection assays with SRF. Expression patterns of representative candidate genes were compared with the LacZ reporter expression activity of the endogenous SRF gene. According to the Unigene data base, 84{\%} of the SRF target candidates were expressed, at least, in the heart. In SRF null embryonic stem cells, 81{\%} of these SRF target candidates were greatly affected by the absence of SRF. Among these SRF-regulated genes, Raf1, Map4k4, and Bicc1 have essential roles in mesoderm formation. The 12 regulated SRF target genes, Mapk10 (JNK3), Txnl2, Azi2, Tera, Sema3a, Lrp4, Actc1, Myl3, Hspg2, Pgm2, Hif3a, and Asb5, have been implicated in cardiovascular formation, and the Ski and Hes6 genes have roles in muscle differentiation. SRF target genes related to cell mitosis and cycle, E2f5, Npm1, Cenpb, Rbbp6, and Scyl1, expressed in the heart tissue were differentially regulated in SRF null ES cells.",
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AU - Zhang, Shu Xing

AU - Garcia-Gras, Eduardo

AU - Wycuff, Diane R.

AU - Marriot, Suzanne J.

AU - Kadeer, Nijiati

AU - Yu, Wei

AU - Olson, Eric N.

AU - Garry, Daniel J.

AU - Parmacek, Michael S.

AU - Schwartz, Robert J.

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