Identification of genes associated with sensitivity to 5-flourouracil and cisplatin in hepatoma cells

Yujin Hoshida, Masaru Moriyama, Motoyuki Otsuka, Naoya Kato, Tadashi Goto, Hiroyoshi Taniguchi, Yasushi Shiratori, Naohiko Seki, Masao Omata

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Background: The prognosis of patients with advanced hepatoma is grim. Although chemotherapy is adapted to such patients, the efficacy is low and the outcome cannot be predicted before therapy. In this study, we aimed to identify genes associated with sensitivity to 5-fluorouracil and cisplatin, drugs widely used in treatment, using gene expression profiles. Methods: Gene expression was evaluated in eight human hepatoma cell lines using an in-house cDNA microarray including 2300 known genes. The 50% growth inhibitory concentrations (GI50) of 5-fluorouracil and cisplatin were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and designated as chemosensitivity. Genes with expression ratios associated with GI50 were selected using the permutation test. Results: For 5-fluorouracil and cisplatin, 21 and 40 genes, respectively, were selected. From among the genes associated with 5-fluorouracil and cisplatin, several encoding metabolic enzymes were selected. In addition, several genes involved in the cell cycle and transcription were identified. Conclusions: We identified genes that may be associated with sensitivity to 5-fluorouracil and cisplatin. A list of these genes may be useful to elucidate how these drugs work on human hepatoma.

Original languageEnglish (US)
Pages (from-to)92-95
Number of pages4
JournalJournal of Gastroenterology
Volume37
Issue numberSUPPL. 14
DOIs
StatePublished - Nov 1 2002
Externally publishedYes

Keywords

  • 5-fluorouracil
  • Chemosensitivity
  • Cisplatin
  • Gene expression

ASJC Scopus subject areas

  • Gastroenterology

Fingerprint Dive into the research topics of 'Identification of genes associated with sensitivity to 5-flourouracil and cisplatin in hepatoma cells'. Together they form a unique fingerprint.

  • Cite this

    Hoshida, Y., Moriyama, M., Otsuka, M., Kato, N., Goto, T., Taniguchi, H., Shiratori, Y., Seki, N., & Omata, M. (2002). Identification of genes associated with sensitivity to 5-flourouracil and cisplatin in hepatoma cells. Journal of Gastroenterology, 37(SUPPL. 14), 92-95. https://doi.org/10.1007/bf03326423