Identification of human melanoma peptides recognized by class I restricted tumor infiltrating T lymphocytes

W. J. Storkus, H. J. Zeh, M. J. Maeurer, R. D. Salter, M. T. Lotze

Research output: Contribution to journalArticle

134 Citations (Scopus)

Abstract

Using a newly described pH 3.3 acid elution technique, peptides were extracted by denaturation of class I molecules on the surface of human melanomas. HPLC fractionation of this material revealed six T cell epitopes (termed P1-P6) recognized by HLA-A2-restricted, melanoma-specific tumor infiltrating lymphocyte (TIL) lines. Three of these fractions (P1, P2, and P4) appeared to represent shared/immunodominant melanoma Ag recognized in the context of HLA-A2 because they were expressed by 4/4 HLA-A2+ melanoma cell lines and were each recognized by all four oligoclonal HLA-A2-restricted TIL lines examined. Interestingly, P1 and P2 (but not P3-P6) could also be recognized by these same TIL when presented by the HLA-Aw69 class I molecule, which is closely related to HLA-A2. P3, P5, and P6 displayed more restricted expression and were differentially recognized by the four oligoclonal TIL lines. These results suggest that synthetic peptide derived from P1, P2, and P4 sequences (when deduced) may form the basis of effective prophylactic or therapeutic melanoma vaccines by stimulating CD8+ CTL in HLA-A2+ individuals. This approach of identifying T cell epitopes presented by class I molecules should prove generally applicable to the study of other tumors recognized by class I-restricted CTL.

Original languageEnglish (US)
Pages (from-to)3719-3727
Number of pages9
JournalJournal of Immunology
Volume151
Issue number7
StatePublished - Jan 1 1993
Externally publishedYes

Fingerprint

Tumor-Infiltrating Lymphocytes
HLA-A2 Antigen
Forensic Anthropology
Melanoma
T-Lymphocytes
Peptides
T-Lymphocyte Epitopes
Vaccines
High Pressure Liquid Chromatography
Cell Line
Acids
Neoplasms

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Identification of human melanoma peptides recognized by class I restricted tumor infiltrating T lymphocytes. / Storkus, W. J.; Zeh, H. J.; Maeurer, M. J.; Salter, R. D.; Lotze, M. T.

In: Journal of Immunology, Vol. 151, No. 7, 01.01.1993, p. 3719-3727.

Research output: Contribution to journalArticle

Storkus, WJ, Zeh, HJ, Maeurer, MJ, Salter, RD & Lotze, MT 1993, 'Identification of human melanoma peptides recognized by class I restricted tumor infiltrating T lymphocytes', Journal of Immunology, vol. 151, no. 7, pp. 3719-3727.
Storkus, W. J. ; Zeh, H. J. ; Maeurer, M. J. ; Salter, R. D. ; Lotze, M. T. / Identification of human melanoma peptides recognized by class I restricted tumor infiltrating T lymphocytes. In: Journal of Immunology. 1993 ; Vol. 151, No. 7. pp. 3719-3727.
@article{1920adfadb294b6d9393906d923bf2aa,
title = "Identification of human melanoma peptides recognized by class I restricted tumor infiltrating T lymphocytes",
abstract = "Using a newly described pH 3.3 acid elution technique, peptides were extracted by denaturation of class I molecules on the surface of human melanomas. HPLC fractionation of this material revealed six T cell epitopes (termed P1-P6) recognized by HLA-A2-restricted, melanoma-specific tumor infiltrating lymphocyte (TIL) lines. Three of these fractions (P1, P2, and P4) appeared to represent shared/immunodominant melanoma Ag recognized in the context of HLA-A2 because they were expressed by 4/4 HLA-A2+ melanoma cell lines and were each recognized by all four oligoclonal HLA-A2-restricted TIL lines examined. Interestingly, P1 and P2 (but not P3-P6) could also be recognized by these same TIL when presented by the HLA-Aw69 class I molecule, which is closely related to HLA-A2. P3, P5, and P6 displayed more restricted expression and were differentially recognized by the four oligoclonal TIL lines. These results suggest that synthetic peptide derived from P1, P2, and P4 sequences (when deduced) may form the basis of effective prophylactic or therapeutic melanoma vaccines by stimulating CD8+ CTL in HLA-A2+ individuals. This approach of identifying T cell epitopes presented by class I molecules should prove generally applicable to the study of other tumors recognized by class I-restricted CTL.",
author = "Storkus, {W. J.} and Zeh, {H. J.} and Maeurer, {M. J.} and Salter, {R. D.} and Lotze, {M. T.}",
year = "1993",
month = "1",
day = "1",
language = "English (US)",
volume = "151",
pages = "3719--3727",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "7",

}

TY - JOUR

T1 - Identification of human melanoma peptides recognized by class I restricted tumor infiltrating T lymphocytes

AU - Storkus, W. J.

AU - Zeh, H. J.

AU - Maeurer, M. J.

AU - Salter, R. D.

AU - Lotze, M. T.

PY - 1993/1/1

Y1 - 1993/1/1

N2 - Using a newly described pH 3.3 acid elution technique, peptides were extracted by denaturation of class I molecules on the surface of human melanomas. HPLC fractionation of this material revealed six T cell epitopes (termed P1-P6) recognized by HLA-A2-restricted, melanoma-specific tumor infiltrating lymphocyte (TIL) lines. Three of these fractions (P1, P2, and P4) appeared to represent shared/immunodominant melanoma Ag recognized in the context of HLA-A2 because they were expressed by 4/4 HLA-A2+ melanoma cell lines and were each recognized by all four oligoclonal HLA-A2-restricted TIL lines examined. Interestingly, P1 and P2 (but not P3-P6) could also be recognized by these same TIL when presented by the HLA-Aw69 class I molecule, which is closely related to HLA-A2. P3, P5, and P6 displayed more restricted expression and were differentially recognized by the four oligoclonal TIL lines. These results suggest that synthetic peptide derived from P1, P2, and P4 sequences (when deduced) may form the basis of effective prophylactic or therapeutic melanoma vaccines by stimulating CD8+ CTL in HLA-A2+ individuals. This approach of identifying T cell epitopes presented by class I molecules should prove generally applicable to the study of other tumors recognized by class I-restricted CTL.

AB - Using a newly described pH 3.3 acid elution technique, peptides were extracted by denaturation of class I molecules on the surface of human melanomas. HPLC fractionation of this material revealed six T cell epitopes (termed P1-P6) recognized by HLA-A2-restricted, melanoma-specific tumor infiltrating lymphocyte (TIL) lines. Three of these fractions (P1, P2, and P4) appeared to represent shared/immunodominant melanoma Ag recognized in the context of HLA-A2 because they were expressed by 4/4 HLA-A2+ melanoma cell lines and were each recognized by all four oligoclonal HLA-A2-restricted TIL lines examined. Interestingly, P1 and P2 (but not P3-P6) could also be recognized by these same TIL when presented by the HLA-Aw69 class I molecule, which is closely related to HLA-A2. P3, P5, and P6 displayed more restricted expression and were differentially recognized by the four oligoclonal TIL lines. These results suggest that synthetic peptide derived from P1, P2, and P4 sequences (when deduced) may form the basis of effective prophylactic or therapeutic melanoma vaccines by stimulating CD8+ CTL in HLA-A2+ individuals. This approach of identifying T cell epitopes presented by class I molecules should prove generally applicable to the study of other tumors recognized by class I-restricted CTL.

UR - http://www.scopus.com/inward/record.url?scp=0027214726&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027214726&partnerID=8YFLogxK

M3 - Article

C2 - 7690811

AN - SCOPUS:0027214726

VL - 151

SP - 3719

EP - 3727

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 7

ER -