Identification of hyaluronan binding proteins using a biotinylated hyaluronan probe

K. Hoare, R. C. Savani, C. Wang, B. Yang, E. A. Turley

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

A method for detecting hyaluronan (HA)-binding proteins in transblot assays using biotinylated HA (BHA) is described. Some of the binding characteristics of a novel HA receptor termed RHAMM (Receptor for HA-Mediated Motility) are characterized using this assay. The method is also used to detect other HA-binding proteins in tissue homogenates. This method is semiquantitative, rapid, reproducible, sensitive and therefore of potential use in identifying the levels of HA-binding proteins in different cells and tissues.

Original languageEnglish (US)
Pages (from-to)117-126
Number of pages10
JournalConnective Tissue Research
Volume30
Issue number2
DOIs
StatePublished - 1993

Keywords

  • Glycosaminoglycans
  • Hyaluronan analytical methods

ASJC Scopus subject areas

  • Rheumatology
  • Biochemistry
  • Orthopedics and Sports Medicine
  • Molecular Biology
  • Cell Biology

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