Identification of hyaluronan binding proteins using a biotinylated hyaluronan probe

K. Hoare; R. C. Savani; C. Wang; B. Yang; E. A. Turley

doi:10.3109/03008209309041327

Identification of hyaluronan binding proteins using a biotinylated hyaluronan probe

K. Hoare, R. C. Savani, C. Wang, B. Yang, E. A. Turley

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

A method for detecting hyaluronan (HA)-binding proteins in transblot assays using biotinylated HA (BHA) is described. Some of the binding characteristics of a novel HA receptor termed RHAMM (Receptor for HA-Mediated Motility) are characterized using this assay. The method is also used to detect other HA-binding proteins in tissue homogenates. This method is semiquantitative, rapid, reproducible, sensitive and therefore of potential use in identifying the levels of HA-binding proteins in different cells and tissues.

Original language	English (US)
Pages (from-to)	117-126
Number of pages	10
Journal	Connective Tissue Research
Volume	30
Issue number	2
DOIs	https://doi.org/10.3109/03008209309041327
State	Published - 1993

Keywords

Glycosaminoglycans
Hyaluronan analytical methods

ASJC Scopus subject areas

Rheumatology
Biochemistry
Orthopedics and Sports Medicine
Molecular Biology
Cell Biology

Access to Document

10.3109/03008209309041327

Cite this

@article{de7028a2d8234d948a1784ac2915f262,

title = "Identification of hyaluronan binding proteins using a biotinylated hyaluronan probe",

abstract = "A method for detecting hyaluronan (HA)-binding proteins in transblot assays using biotinylated HA (BHA) is described. Some of the binding characteristics of a novel HA receptor termed RHAMM (Receptor for HA-Mediated Motility) are characterized using this assay. The method is also used to detect other HA-binding proteins in tissue homogenates. This method is semiquantitative, rapid, reproducible, sensitive and therefore of potential use in identifying the levels of HA-binding proteins in different cells and tissues.",

keywords = "Glycosaminoglycans, Hyaluronan analytical methods",

author = "K. Hoare and Savani, {R. C.} and C. Wang and B. Yang and Turley, {E. A.}",

note = "Funding Information: This work was funded by an NClC grant and NIH grant (CA #51540) to E.T. E.T. is a recipient of a Children{\textquoteright}s Hospital Research Foundation Scholarship.",

year = "1993",

doi = "10.3109/03008209309041327",

language = "English (US)",

volume = "30",

pages = "117--126",

journal = "Connective Tissue Research",

issn = "0300-8207",

publisher = "Informa Healthcare",

number = "2",

}

TY - JOUR

T1 - Identification of hyaluronan binding proteins using a biotinylated hyaluronan probe

AU - Hoare, K.

AU - Savani, R. C.

AU - Wang, C.

AU - Yang, B.

AU - Turley, E. A.

N1 - Funding Information: This work was funded by an NClC grant and NIH grant (CA #51540) to E.T. E.T. is a recipient of a Children’s Hospital Research Foundation Scholarship.

PY - 1993

Y1 - 1993

N2 - A method for detecting hyaluronan (HA)-binding proteins in transblot assays using biotinylated HA (BHA) is described. Some of the binding characteristics of a novel HA receptor termed RHAMM (Receptor for HA-Mediated Motility) are characterized using this assay. The method is also used to detect other HA-binding proteins in tissue homogenates. This method is semiquantitative, rapid, reproducible, sensitive and therefore of potential use in identifying the levels of HA-binding proteins in different cells and tissues.

AB - A method for detecting hyaluronan (HA)-binding proteins in transblot assays using biotinylated HA (BHA) is described. Some of the binding characteristics of a novel HA receptor termed RHAMM (Receptor for HA-Mediated Motility) are characterized using this assay. The method is also used to detect other HA-binding proteins in tissue homogenates. This method is semiquantitative, rapid, reproducible, sensitive and therefore of potential use in identifying the levels of HA-binding proteins in different cells and tissues.

KW - Glycosaminoglycans

KW - Hyaluronan analytical methods

UR - http://www.scopus.com/inward/record.url?scp=0027146250&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027146250&partnerID=8YFLogxK

U2 - 10.3109/03008209309041327

DO - 10.3109/03008209309041327

M3 - Article

C2 - 7512013

AN - SCOPUS:0027146250

SN - 0300-8207

VL - 30

SP - 117

EP - 126

JO - Connective Tissue Research

JF - Connective Tissue Research

IS - 2

ER -

Identification of hyaluronan binding proteins using a biotinylated hyaluronan probe

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this