Identification of hyperthyroid patients by means of a sensitive assay for thyrotropin

J. S. Malter, S. E. Manotti, G. R. Knee, D. B. Goodman, J. F. Strauss

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We evaluated a new assay (TSH3 MAIAclone) for thyrotropin (TSH) with improved sensitivity, testing a series of hospital inpatients with increased free thyroxin indices (thyroxin concentration x triiodothyronine uptake on resin). This assay involves use of three monoclonal antibodies and an antibody-magnetic particle conjugate that rapidly and completely separates bound and free tracer in a magnetic field. The assay turnaround time is 3 h. By the TSH3-MAIAclone assay, 65% of these patients with an increased free thyroxin index were identified on the basis of a TSH value ≤0.50 milli-int. unit/L. In contrast, another commercially available assay for TSH detected suppressed TSH concentrations in less than 5% of these patients. We conclude that the TSH3 MAIAclone assay markedly improves our ability to discriminate hyperthyroidism from euthyroidism.

Original languageEnglish (US)
Pages (from-to)642-644
Number of pages3
JournalClinical Chemistry
Volume31
Issue number4
StatePublished - 1985

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Thyrotropin
Hyperthyroidism
Thyroxine
Assays
Triiodothyronine
Magnetic Fields
Inpatients
Monoclonal Antibodies
Turnaround time
Antibodies
Resins
Magnetic fields
Testing

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Malter, J. S., Manotti, S. E., Knee, G. R., Goodman, D. B., & Strauss, J. F. (1985). Identification of hyperthyroid patients by means of a sensitive assay for thyrotropin. Clinical Chemistry, 31(4), 642-644.

Identification of hyperthyroid patients by means of a sensitive assay for thyrotropin. / Malter, J. S.; Manotti, S. E.; Knee, G. R.; Goodman, D. B.; Strauss, J. F.

In: Clinical Chemistry, Vol. 31, No. 4, 1985, p. 642-644.

Research output: Contribution to journalArticle

Malter, JS, Manotti, SE, Knee, GR, Goodman, DB & Strauss, JF 1985, 'Identification of hyperthyroid patients by means of a sensitive assay for thyrotropin', Clinical Chemistry, vol. 31, no. 4, pp. 642-644.
Malter, J. S. ; Manotti, S. E. ; Knee, G. R. ; Goodman, D. B. ; Strauss, J. F. / Identification of hyperthyroid patients by means of a sensitive assay for thyrotropin. In: Clinical Chemistry. 1985 ; Vol. 31, No. 4. pp. 642-644.
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