Identification of Leptin Receptor-Expressing Cells in the Nodose Ganglion of Male Mice

Luis Leon Mercado, Alexandre Caron, Yibing Wang, Michael Burton, Laurent Gautron

Research output: Contribution to journalArticle

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Abstract

Leptin has been proposed to modulate viscerosensory information directly at the level of vagal afferents. In support of this view, broad expression for the leptin receptor (Lepr) has previously been reported in vagal afferents. However, the exact identity and distribution of leptin-sensitive vagal afferents has not been elucidated. Using quantitative PCR, we found that the whole mouse nodose ganglion was predominantly enriched in the short form of Lepr, rather than its long form. Consistent with this observation, the acute administration of leptin did not stimulate JAK-STAT signaling in the nodose ganglion. Using chromogenic in situ hybridization in wild-type mice and several reporter mouse models, we demonstrated that Lepr mRNA was restricted to nonneuronal cells in the epineurium and parenchyma of the nodose ganglion and a subset of vagal afferents, which accounted for only 3% of all neuronal profiles. Double labeling studies further established that Lepr-expressing vagal afferents were Nav1.8-negative fibers that did not supply the peritoneal cavity. Finally, double chromogenic in situ hybridization revealed that many Lepr-expressing neurons coexpressed the angiotensin 1a receptor (At1ar), which is a gene expressed in baroreceptors. Taken together, our data challenge the commonly held view that Lepr is broadly expressed in vagal afferents. Instead, our data suggest that leptin may exert a previously unrecognized role, mainly via its short form, as a direct modulator of a very small group of At1ar-positive vagal fibers.

Original languageEnglish (US)
Pages (from-to)1307-1322
Number of pages16
JournalEndocrinology
Volume160
Issue number5
DOIs
StatePublished - May 1 2019

Fingerprint

Nodose Ganglion
Leptin Receptors
Leptin
Angiotensin Receptors
In Situ Hybridization
Pressoreceptors
Peritoneal Cavity
Peripheral Nerves
Neurons
Polymerase Chain Reaction
Messenger RNA
Genes

ASJC Scopus subject areas

  • Endocrinology

Cite this

Identification of Leptin Receptor-Expressing Cells in the Nodose Ganglion of Male Mice. / Leon Mercado, Luis; Caron, Alexandre; Wang, Yibing; Burton, Michael; Gautron, Laurent.

In: Endocrinology, Vol. 160, No. 5, 01.05.2019, p. 1307-1322.

Research output: Contribution to journalArticle

Leon Mercado, Luis ; Caron, Alexandre ; Wang, Yibing ; Burton, Michael ; Gautron, Laurent. / Identification of Leptin Receptor-Expressing Cells in the Nodose Ganglion of Male Mice. In: Endocrinology. 2019 ; Vol. 160, No. 5. pp. 1307-1322.
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