Identification of macromolecular complexes in cryoelectron tomograms of phantom cells

Achilleas S. Frangakis, Jochen Böhm, Friedrich Förster, Stephan Nickell, Daniela Nicastro, Dieter Typke, Reiner Hegerl, Wolfgang Baumeister

Research output: Contribution to journalArticle

183 Citations (Scopus)

Abstract

Electron tomograms of intact frozen-hydrated cells are essentially three-dimensional images of the entire proteome of the cell, and they depict the whole network of macromolecular interactions. However, this information is not easily accessible because of the poor signal-to-noise ratio of the tomograms and the crowded nature of the cytoplasm. Here, we describe a template matching algorithm that is capable of detecting and identifying macromolecules in tomographic volumes in a fully automated manner. The algorithm is based on nonlinear cross correlation and incorporates elements of multivariate statistical analysis. Phantom cells, i.e., lipid vesicles filled with macromolecules, provide a realistic experimental scenario for an assessment of the fidelity of this approach. At the current resolution of ≈4 nm, macromolecules in the size range of 0.5-1 MDa can be identified with good fidelity.

Original languageEnglish (US)
Pages (from-to)14153-14158
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume99
Issue number22
DOIs
StatePublished - Oct 29 2002

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Macromolecular Substances
Three-Dimensional Imaging
Signal-To-Noise Ratio
Proteome
Cytoplasm
Multivariate Analysis
Electrons
Lipids

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Identification of macromolecular complexes in cryoelectron tomograms of phantom cells. / Frangakis, Achilleas S.; Böhm, Jochen; Förster, Friedrich; Nickell, Stephan; Nicastro, Daniela; Typke, Dieter; Hegerl, Reiner; Baumeister, Wolfgang.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, No. 22, 29.10.2002, p. 14153-14158.

Research output: Contribution to journalArticle

Frangakis, Achilleas S. ; Böhm, Jochen ; Förster, Friedrich ; Nickell, Stephan ; Nicastro, Daniela ; Typke, Dieter ; Hegerl, Reiner ; Baumeister, Wolfgang. / Identification of macromolecular complexes in cryoelectron tomograms of phantom cells. In: Proceedings of the National Academy of Sciences of the United States of America. 2002 ; Vol. 99, No. 22. pp. 14153-14158.
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