Abstract
MHC class I-related chain A (MICA) is an HLA-related, polymorphic gene the product of which may be recognized by a subpopularion of intestinal γδ T cells and may play a role in the activation of a subpopulation of natural killer cells. Using anti-MICA specific rabbit sera we previously demonstrated that freshly isolated monocytes, keratinocytes, fibroblasts, and endothelial cells express MICA. To analyze whether MICA may be a target for specific antibodies in sera of transplanted patients, we produced three recombinant MICA proteins consisting of the α1, α2, and α3 domains, and used them in an enzyme-linked immunosorbent assay. We found that several patients had specific antibodies against MICA. Most of them were detected in serum samples collected at different times after organ rejection. Although this finding raises the question of how these patients became immunized, the fact that the polymorphic, HLA-like MICA molecule, expressed at the cell surface of endothelial cells, is recognized by specific antibodies in sera of transplanted patients, suggests the MICA may be a target molecule in allograft rejection. (C) American Society for Histocompatibility and Immunogenetics, 2000.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 917-924 |
| Number of pages | 8 |
| Journal | Human Immunology |
| Volume | 61 |
| Issue number | 9 |
| DOIs | |
| State | Published - 2000 |
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Keywords
- Alioantigen
- HLA
- MICA
- Transplantation
ASJC Scopus subject areas
- Immunology
- Immunology and Allergy
Cite this
Identification of MICA as a new polymorphic alloantigen recognized by antibodies in sera of organ transplant recipients. / Zwirner, Norberto W.; Marcos, Cintia Y.; Mirbaha, Fariba; Zou, Yizhou; Stastny, Peter.
In: Human Immunology, Vol. 61, No. 9, 2000, p. 917-924.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification of MICA as a new polymorphic alloantigen recognized by antibodies in sera of organ transplant recipients
AU - Zwirner, Norberto W.
AU - Marcos, Cintia Y.
AU - Mirbaha, Fariba
AU - Zou, Yizhou
AU - Stastny, Peter
PY - 2000
Y1 - 2000
N2 - MHC class I-related chain A (MICA) is an HLA-related, polymorphic gene the product of which may be recognized by a subpopularion of intestinal γδ T cells and may play a role in the activation of a subpopulation of natural killer cells. Using anti-MICA specific rabbit sera we previously demonstrated that freshly isolated monocytes, keratinocytes, fibroblasts, and endothelial cells express MICA. To analyze whether MICA may be a target for specific antibodies in sera of transplanted patients, we produced three recombinant MICA proteins consisting of the α1, α2, and α3 domains, and used them in an enzyme-linked immunosorbent assay. We found that several patients had specific antibodies against MICA. Most of them were detected in serum samples collected at different times after organ rejection. Although this finding raises the question of how these patients became immunized, the fact that the polymorphic, HLA-like MICA molecule, expressed at the cell surface of endothelial cells, is recognized by specific antibodies in sera of transplanted patients, suggests the MICA may be a target molecule in allograft rejection. (C) American Society for Histocompatibility and Immunogenetics, 2000.
AB - MHC class I-related chain A (MICA) is an HLA-related, polymorphic gene the product of which may be recognized by a subpopularion of intestinal γδ T cells and may play a role in the activation of a subpopulation of natural killer cells. Using anti-MICA specific rabbit sera we previously demonstrated that freshly isolated monocytes, keratinocytes, fibroblasts, and endothelial cells express MICA. To analyze whether MICA may be a target for specific antibodies in sera of transplanted patients, we produced three recombinant MICA proteins consisting of the α1, α2, and α3 domains, and used them in an enzyme-linked immunosorbent assay. We found that several patients had specific antibodies against MICA. Most of them were detected in serum samples collected at different times after organ rejection. Although this finding raises the question of how these patients became immunized, the fact that the polymorphic, HLA-like MICA molecule, expressed at the cell surface of endothelial cells, is recognized by specific antibodies in sera of transplanted patients, suggests the MICA may be a target molecule in allograft rejection. (C) American Society for Histocompatibility and Immunogenetics, 2000.
KW - Alioantigen
KW - HLA
KW - MICA
KW - Transplantation
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U2 - 10.1016/S0198-8859(00)00162-2
DO - 10.1016/S0198-8859(00)00162-2
M3 - Article
VL - 61
SP - 917
EP - 924
JO - Human Immunology
JF - Human Immunology
SN - 0198-8859
IS - 9
ER -