Identification of morc (microrchidia), a mutation that results in arrest of spermatogenesis at an early meiotic stage in the mouse

M. L. Watson, A. R. Zinn, N. Inoue, K. D. Hess, J. Cobb, M. A. Handel, R. Halaban, C. C. Duchene, G. M. Albright, R. W. Moreadith

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Abstract

The microrchidia, or more, autosomal recessive mutation results in the arrest of spermatogenesis early in prophase I of meiosis. The more mutation arose spontaneously during the development of a mouse strain transgenic for a tyrosinase cDNA construct. Morc-/- males are infertile and have grossly reduced testicular mass, whereas -/- females are normal, indicating that the Morc gene acts specifically during male gametogenesis. Immunofluorescence to synaptonemal complex antigens demonstrated that -/- male germ cells enter meiosis but fail to progress beyond zygotene or leptotene stage. An apoptosis assay revealed massive numbers of cells undergoing apoptosis in testes of - /- mice. No other abnormal phenotype was observed in mutant animals, with the exception of eye pigmentation caused by transgene expression in the retina. Spermatogenesis is normal in +/- males, despite significant transgene expression in germ cells. Genomic analysis of -/- animals indicates the presence of a deletion adjacent to the transgene. Identification of the gene inactivated by the transgene insertion may define a novel biochemical pathway involved in mammalian germ cell development and meiosis.

Original languageEnglish (US)
Pages (from-to)14361-14366
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume95
Issue number24
DOIs
StatePublished - Nov 24 1998

Fingerprint

Meiotic Prophase I
Transgenes
Meiosis
Germ Cells
Mutation
Synaptonemal Complex
Apoptosis
Gametogenesis
Monophenol Monooxygenase
Pigmentation
Spermatogenesis
Transgenic Mice
Genes
Fluorescent Antibody Technique
Retina
Testis
Complementary DNA
Cell Count
Phenotype
Antigens

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Identification of morc (microrchidia), a mutation that results in arrest of spermatogenesis at an early meiotic stage in the mouse. / Watson, M. L.; Zinn, A. R.; Inoue, N.; Hess, K. D.; Cobb, J.; Handel, M. A.; Halaban, R.; Duchene, C. C.; Albright, G. M.; Moreadith, R. W.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, No. 24, 24.11.1998, p. 14361-14366.

Research output: Contribution to journalArticle

Watson, M. L. ; Zinn, A. R. ; Inoue, N. ; Hess, K. D. ; Cobb, J. ; Handel, M. A. ; Halaban, R. ; Duchene, C. C. ; Albright, G. M. ; Moreadith, R. W. / Identification of morc (microrchidia), a mutation that results in arrest of spermatogenesis at an early meiotic stage in the mouse. In: Proceedings of the National Academy of Sciences of the United States of America. 1998 ; Vol. 95, No. 24. pp. 14361-14366.
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AU - Hess, K. D.

AU - Cobb, J.

AU - Handel, M. A.

AU - Halaban, R.

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AU - Albright, G. M.

AU - Moreadith, R. W.

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