TY - JOUR
T1 - Identification of New Subunits of the Multiprotein Mammalian TRRAP/TIP60-containing Histone Acetyltransferase Complex
AU - Cai, Yong
AU - Jin, Jingji
AU - Tomomori-Sato, Chieri
AU - Sato, Shigeo
AU - Sorokina, Irina
AU - Parmely, Tari J.
AU - Conaway, Ronald C.
AU - Conaway, Joan Weliky
PY - 2003/10/31
Y1 - 2003/10/31
N2 - The mammalian ATM/PI 3-kinase-related TRRAP protein was previously found to be a component of a multiprotein histone acetyltransferase (HAT) complex containing the HAT TIP60. In this report, we identify a previously uncharacterized protein encoded by the FLJ10914 ORF, which we designate MRGBP, as a new component of the TRRAP/TIP60 HAT complex. In addition, through purification of MRGBP and its associated proteins from HeLa cell nuclear extracts, we identify the thyroid receptor coactivating protein (TRCp120), DMAP1, and the related MRG15 and MRGX proteins as MRGBP-associating proteins, and we present biochemical evidence that they are previously unrecognized components of the TRRAP/TIP60 HAT complex. Taken together, our findings shed new light on the structure and function of the mammalian TRRAP/TIP60 histone acetyltransferase complex.
AB - The mammalian ATM/PI 3-kinase-related TRRAP protein was previously found to be a component of a multiprotein histone acetyltransferase (HAT) complex containing the HAT TIP60. In this report, we identify a previously uncharacterized protein encoded by the FLJ10914 ORF, which we designate MRGBP, as a new component of the TRRAP/TIP60 HAT complex. In addition, through purification of MRGBP and its associated proteins from HeLa cell nuclear extracts, we identify the thyroid receptor coactivating protein (TRCp120), DMAP1, and the related MRG15 and MRGX proteins as MRGBP-associating proteins, and we present biochemical evidence that they are previously unrecognized components of the TRRAP/TIP60 HAT complex. Taken together, our findings shed new light on the structure and function of the mammalian TRRAP/TIP60 histone acetyltransferase complex.
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U2 - 10.1074/jbc.C300389200
DO - 10.1074/jbc.C300389200
M3 - Article
C2 - 12963728
AN - SCOPUS:0242322038
SN - 0021-9258
VL - 278
SP - 42733
EP - 42736
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 44
ER -