Abstract
We evaluated the Bruker Biotyper matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry for identification of 92 clinical isolates of Corynebacterium species in comparison to identification using rpoB or 16S rRNA gene sequencing. Eighty isolates (87%) yielded a score of≥1.700, and all of these were correctly identified to the species level with the exception of Corynebacterium aurimucosum being misidentified as the closely related Corynebacterium minutissimum.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 160-163 |
| Number of pages | 4 |
| Journal | Journal of Clinical Microbiology |
| Volume | 50 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 2012 |
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ASJC Scopus subject areas
- Microbiology (medical)
Cite this
Identification of non-diphtheriae Corynebacterium by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry. / Alatoom, Adnan A.; Cazanave, Charles J.; Cunningham, Scott A.; Ihde, Sherry M.; Patela, Robin.
In: Journal of Clinical Microbiology, Vol. 50, No. 1, 01.2012, p. 160-163.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Identification of non-diphtheriae Corynebacterium by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry
AU - Alatoom, Adnan A.
AU - Cazanave, Charles J.
AU - Cunningham, Scott A.
AU - Ihde, Sherry M.
AU - Patela, Robin
PY - 2012/1
Y1 - 2012/1
N2 - We evaluated the Bruker Biotyper matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry for identification of 92 clinical isolates of Corynebacterium species in comparison to identification using rpoB or 16S rRNA gene sequencing. Eighty isolates (87%) yielded a score of≥1.700, and all of these were correctly identified to the species level with the exception of Corynebacterium aurimucosum being misidentified as the closely related Corynebacterium minutissimum.
AB - We evaluated the Bruker Biotyper matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry for identification of 92 clinical isolates of Corynebacterium species in comparison to identification using rpoB or 16S rRNA gene sequencing. Eighty isolates (87%) yielded a score of≥1.700, and all of these were correctly identified to the species level with the exception of Corynebacterium aurimucosum being misidentified as the closely related Corynebacterium minutissimum.
UR - http://www.scopus.com/inward/record.url?scp=84555189792&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84555189792&partnerID=8YFLogxK
U2 - 10.1128/JCM.05889-11
DO - 10.1128/JCM.05889-11
M3 - Article
C2 - 22075579
AN - SCOPUS:84555189792
VL - 50
SP - 160
EP - 163
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
SN - 0095-1137
IS - 1
ER -