Identification of non-diphtheriae Corynebacterium by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry

Adnan A. Alatoom, Charles J. Cazanave, Scott A. Cunningham, Sherry M. Ihde, Robin Patela

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

We evaluated the Bruker Biotyper matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry for identification of 92 clinical isolates of Corynebacterium species in comparison to identification using rpoB or 16S rRNA gene sequencing. Eighty isolates (87%) yielded a score of≥1.700, and all of these were correctly identified to the species level with the exception of Corynebacterium aurimucosum being misidentified as the closely related Corynebacterium minutissimum.

Original languageEnglish (US)
Pages (from-to)160-163
Number of pages4
JournalJournal of Clinical Microbiology
Volume50
Issue number1
DOIs
StatePublished - Jan 2012

Fingerprint

Corynebacterium
Mass Spectrometry
Lasers
rRNA Genes

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Identification of non-diphtheriae Corynebacterium by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry. / Alatoom, Adnan A.; Cazanave, Charles J.; Cunningham, Scott A.; Ihde, Sherry M.; Patela, Robin.

In: Journal of Clinical Microbiology, Vol. 50, No. 1, 01.2012, p. 160-163.

Research output: Contribution to journalArticle

Alatoom, Adnan A. ; Cazanave, Charles J. ; Cunningham, Scott A. ; Ihde, Sherry M. ; Patela, Robin. / Identification of non-diphtheriae Corynebacterium by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry. In: Journal of Clinical Microbiology. 2012 ; Vol. 50, No. 1. pp. 160-163.
@article{5fdde155dee449e480a26e3eb8d285ea,
title = "Identification of non-diphtheriae Corynebacterium by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry",
abstract = "We evaluated the Bruker Biotyper matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry for identification of 92 clinical isolates of Corynebacterium species in comparison to identification using rpoB or 16S rRNA gene sequencing. Eighty isolates (87{\%}) yielded a score of≥1.700, and all of these were correctly identified to the species level with the exception of Corynebacterium aurimucosum being misidentified as the closely related Corynebacterium minutissimum.",
author = "Alatoom, {Adnan A.} and Cazanave, {Charles J.} and Cunningham, {Scott A.} and Ihde, {Sherry M.} and Robin Patela",
year = "2012",
month = "1",
doi = "10.1128/JCM.05889-11",
language = "English (US)",
volume = "50",
pages = "160--163",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "1",

}

TY - JOUR

T1 - Identification of non-diphtheriae Corynebacterium by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry

AU - Alatoom, Adnan A.

AU - Cazanave, Charles J.

AU - Cunningham, Scott A.

AU - Ihde, Sherry M.

AU - Patela, Robin

PY - 2012/1

Y1 - 2012/1

N2 - We evaluated the Bruker Biotyper matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry for identification of 92 clinical isolates of Corynebacterium species in comparison to identification using rpoB or 16S rRNA gene sequencing. Eighty isolates (87%) yielded a score of≥1.700, and all of these were correctly identified to the species level with the exception of Corynebacterium aurimucosum being misidentified as the closely related Corynebacterium minutissimum.

AB - We evaluated the Bruker Biotyper matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry for identification of 92 clinical isolates of Corynebacterium species in comparison to identification using rpoB or 16S rRNA gene sequencing. Eighty isolates (87%) yielded a score of≥1.700, and all of these were correctly identified to the species level with the exception of Corynebacterium aurimucosum being misidentified as the closely related Corynebacterium minutissimum.

UR - http://www.scopus.com/inward/record.url?scp=84555189792&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84555189792&partnerID=8YFLogxK

U2 - 10.1128/JCM.05889-11

DO - 10.1128/JCM.05889-11

M3 - Article

C2 - 22075579

AN - SCOPUS:84555189792

VL - 50

SP - 160

EP - 163

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 1

ER -