Identification of non-diphtheriae Corynebacterium by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry

Adnan A. Alatoom; Charles J. Cazanave; Scott A. Cunningham; Sherry M. Ihde; Robin Patela

doi:10.1128/JCM.05889-11

Identification of non-diphtheriae Corynebacterium by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry

Adnan A. Alatoom, Charles J. Cazanave, Scott A. Cunningham, Sherry M. Ihde, Robin Patela

Pathology

Research output: Contribution to journal › Article

71 Scopus citations

Abstract

We evaluated the Bruker Biotyper matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry for identification of 92 clinical isolates of Corynebacterium species in comparison to identification using rpoB or 16S rRNA gene sequencing. Eighty isolates (87%) yielded a score of≥1.700, and all of these were correctly identified to the species level with the exception of Corynebacterium aurimucosum being misidentified as the closely related Corynebacterium minutissimum.

Original language	English (US)
Pages (from-to)	160-163
Number of pages	4
Journal	Journal of clinical microbiology
Volume	50
Issue number	1
DOIs	https://doi.org/10.1128/JCM.05889-11
State	Published - Jan 1 2012

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ASJC Scopus subject areas

Microbiology (medical)

Cite this

Alatoom, A. A., Cazanave, C. J., Cunningham, S. A., Ihde, S. M., & Patela, R. (2012). Identification of non-diphtheriae Corynebacterium by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry. Journal of clinical microbiology, 50(1), 160-163. https://doi.org/10.1128/JCM.05889-11

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10.1128/JCM.05889-11