Identification of nonviable muscle in electric burns with nitroblue tetrazolium

John L. Hunt, Ellen L. Heck

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Both experimental and clinical evaluation of nitroblue tetrazolium (NBT) as a method of identifying ischemic and necrotic muscle is described. Colorless NBT was reduced to a blue formazin by viable muscle but remained colorless in necrotic muscle. Muscle samples were rated for viability on a scale of 0-100% based on the relative amount of muscle sample that reduced NBT. There was good correlation between gross appearance, distribution of staining, and histologic findings in all nonviable experimental and clinical muscle specimens. All tissue that reacted with NBT proved to be viable histologically. Metabolically active or viable muscle fibers are rich in respiratory enzymes (dehydrogenases). The loss of dehydrogenase activity from ischemic or necrotic muscle can be detected by NBT reduction capacity. NBT technique identified nonviable tissue, clearly showed the even distribution of muscle damage characteristic of electric burns, was useful in defining ischemic muscle prior to obvious necrosis, and was a rapid, simple, and reliable semiquantitative test that can be used intraoperatively.

Original languageEnglish (US)
Pages (from-to)369-375
Number of pages7
JournalJournal of Surgical Research
Volume37
Issue number5
DOIs
StatePublished - 1984

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Electric Burns
Nitroblue Tetrazolium
Muscles
Oxidoreductases

ASJC Scopus subject areas

  • Surgery

Cite this

Identification of nonviable muscle in electric burns with nitroblue tetrazolium. / Hunt, John L.; Heck, Ellen L.

In: Journal of Surgical Research, Vol. 37, No. 5, 1984, p. 369-375.

Research output: Contribution to journalArticle

Hunt, John L. ; Heck, Ellen L. / Identification of nonviable muscle in electric burns with nitroblue tetrazolium. In: Journal of Surgical Research. 1984 ; Vol. 37, No. 5. pp. 369-375.
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