Identification of novel membrane and secreted proteins upregulated during adipocyte differentiation

Hiromichi Tsuruga, Hidetoshi Kumagai, Tetsuo Kojima, Toshio Kitamura

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Adipose tissue is the largest organ in the body that secretes soluble proteins such as cytokines. A preadipocyte cell line 3T3-L1 has been widely used for investigations of mechanisms of adipocyte differentiation. 3T3-L1 cells convert to adipocytes in the presence of 1-methyl-3-isobutylxanthine, dexamethasone, and insulin. We screened a cDNA library derived from differentiated 3T3-L1 cells, using the SST-REX method (signal sequence trap by retrovirus-mediated expression screening method). Screening of 4 x 105 clones gave rise to 63 known and 8 novel clones. The known clones represented 28 independent proteins, 21 of which were secreted proteins and 7 were membrane proteins. The novel clones represented 7 independent proteins, 5 of which had no similarity to known proteins. Interestingly, most of these novel genes showed differentiation- and tissue-specific expression. The present results indicate that adipocytes specific genes or adipocyte differentiation-related genes encoding membrane and secreted proteins can be readily identified if signal sequence trap screening of differentiated adipocyte-derived cDNAs is done. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)293-297
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume272
Issue number1
DOIs
StatePublished - May 27 2000

Keywords

  • 3T3-L1
  • Adipocyte
  • Differentiation
  • Retrovirus cDNA library
  • Signal sequence trap

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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